Sökning: "TECHNOLOGY Bioengineering"

Visar resultat 16 - 20 av 88 avhandlingar innehållade orden TECHNOLOGY Bioengineering.

1. 16. Membrane-Assisted Isoform ImmunoAssay : Separation and determination of protein isoforms

   Författare :Maria Lönnberg; Carl G M Magnusson; Uppsala universitet; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biotechnology; Bioteknik; Bioengineering; Bioteknik; Surface Biotechnology; ytbioteknik;

   Sammanfattning : Proteins exist in a variety of isoforms with minor differences, mostly due to their glycosylation patterns, which can modulate their biological functions. It seems to be of clinical relevance to measure the isoform-distribution.Thesis describes a novel technology named Membrane-Assisted Isoform ImmunoAssay (MAIIA). LÄS MER

3. 17. Orientation and Conformation of Single Macromolecules on Unmodified and Functionalized Surfaces

   Författare :Magnus Bergkvist; David Cullen; Uppsala universitet; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biotechnology; Atomic Force Microscopy; protein orientation; protein adsorption; surface derivatization; Bioteknik; Bioengineering; Bioteknik; Surface Biotechnology; ytbioteknik;

   Sammanfattning : In this thesis methods for investigation of orientation and conformation of individual macromolecules on surfaces are presented as well as novel methods for functionalization of silicon chips with the possibility to get an ordered immobilization of antibodies. Two novel methods are presented which makes it possible to investigate the orientation of individual macromolecules on different kinds of surfaces with AFM. LÄS MER

4. 18. Direct observation of biomolecule adsorption and spatial distribution of functional groups in chromatographic adsorbent particles

   Författare :Anders Ljunglöf; Abraham Lenhoff; Uppsala universitet; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biotechnology; Confocal microscopy; confocal Raman spectroscopy; protein adsorption; ligand distribution; visualization; imaging; Bioteknik; Bioengineering; Bioteknik; Surface Biotechnology; ytbioteknik;

   Sammanfattning : Confocal microscopy has been used as a tool for studying adsorption of biomolecules to individual chromatographic adsorbent particles. By coupling a fluorescent dye to protein molecules, their penetration into single adsorbent particles could be observed visually at different times during batch uptake. LÄS MER

5. 19. Spatially Controlled Covalent Immobilization of Biomolecules on Silicon Surfaces

   Författare :Elisabeth Pavlovic; Sven Oscarsson; Marcus Textor; Uppsala universitet; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biotechnology; silicon oxide; electrooxidation; sulfur; nanotechnology; self-assembled monolayers; atomic force microscopy; electron spectroscopy for chemical analysis; biomolecules; covalent immobilization; reversibility; Bioteknik; Bioengineering; Bioteknik;

   Sammanfattning : The work described in this thesis aims to achieving surface patterning through chemical activation of thiolated silicon oxide surfaces, resulting in a spatially controlled covalent immobilization of biomolecules with high resolution.Existing chemical methods to immobilize molecules on surfaces do not reach below the micrometer scale while the ones allowing for spatial control mostly lead to non-covalent adsorption of molecules on surfaces, or require several successive chemical reactions to obtain the final covalent immobilization. LÄS MER

7. 20. Endoglucanase and Mannanase from Blue Mussel, Mytilus edulis: Purification, Characterization, Gene and Three Dimensional Structure

   Författare :Bingze Xu; Folke Tjerneld; Uppsala universitet; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biotechnology; Bioteknik; Bioengineering; Bioteknik; Surface Biotechnology; ytbioteknik;

   Sammanfattning : Two polysaccharide-degrading enzymes (endo-1,4-D-glucanase and β-mannanase) from blue mussel, Mytilus edulis, have been purified to homogeneity using a combination of several chromatographic steps. Each enzyme has been characterized with regard to its molecular weight, isoelectric point, pH and temperature stability, pH and temperature optimum and substrate specificity. LÄS MER