Sökning: "Subcellular organelles"

Visar resultat 1 - 5 av 20 avhandlingar innehållade orden Subcellular organelles.

  1. 1. Antibody-based subcellular localization of the human proteome

    Författare :Marie Skogs; Emma Lundberg; Hans Blom; Cecilia Williams; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Human proteome; Subcellular localization; Organelles; Immunofluorescence; Fixation; Permeabilization; Antibody validation; Bioteknologi; Biotechnology;

    Sammanfattning : This thesis describes the use of antibodies and immunofluorescence for subcellular localization of proteins. The key objective is the creation of an open-source atlas with information on the subcellular location of every human protein. LÄS MER

  3. 2. Membrane Proteins in Human Neutrophils - Identification and Characterization of Lipid Rafts in Subcellular Organelles

    Författare :Elisabeth Feuk-Lagerstedt; Göteborgs universitet; []
    Nyckelord :Human neutrophils; Subcellular organelles; Membrane proteins; Lipid rafts; DRMs;

    Sammanfattning : The human neutrophil is an important effector cell in acute inflammation and in the innate immune response against bacteria and fungi. When immune reactions occur in the tissue in response to antigen challenge, neutrophils are the first cells to enter the site of inflammation. LÄS MER

  4. 3. Towards subcellular localization of the human proteome using bioimaging

    Författare :Charlotte Stadler; Emma Lundberg; Carolina Wählby; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Antibody; antibody validation; automated image analysis; automated microscopy; cell line; confocal microscopy; fixation; green fluorescent protein GFP ; immunofluorescence IF ; organelle; protein expression; siRNA; subcellular localization;

    Sammanfattning : Since the publication of the complete sequence of the human genome in 2003 there has been great interest in exploring the functions of the proteins encoded by the genes. To reveal the function of each and every protein, investigation of protein localization at the subcellular level has become a central focus in this research area, since the localization and function of a protein is closely related. LÄS MER

  5. 4. The function of annexin I in human neutrophils. Studies of membrane binding

    Författare :Charlotta Movitz; Göteborgs universitet; []
    Nyckelord :Human neutrophils; secretion; annexin I; grancalcin; subcellular organelles; lipid rafts; DIM;

    Sammanfattning : The neutrophil granulocyte plays an important role in host defense against infections induced by invading microorganisms. The defense mechanism is dependent on the migration of neutrophils from the blood stream to the infected area, where the intruder is phagocytosed and killed. LÄS MER

  7. 5. Spatial proteome profiling of the compartments of the human cell using an antibody-based approach

    Författare :Mikaela Wiking; Emma Lundberg; Björn Önfelt; Torbjörn Gräslund; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Fluorescence imaging; Human proteome; Human Protein Atlas; Immunofluorescence; Mitochondria; Organelles; Spatial distribution; Bioteknologi; Biotechnology;

    Sammanfattning : The human cell is complex, with countless processes ongoing in parallel in specialized compartments, the organelles. Cells can be studied in vitro by using immortalized cell lines that represent cells in vivo to a varying degree. LÄS MER