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Visar resultat 1 - 5 av 11 avhandlingar som matchar ovanstående sökkriterier.

1. 1. Structural and biochemical basis for the high fidelity and processivity of DNA polymerase ε

   Författare :Rais Ahmad Ganai; Erik Johansson; Per Elias; Umeå universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; DNA polymerase ε; Crystal structure; P domain; Pre-steady state kinetics; β-hairpin loop; Primer switching; Strand displacement; biokemi; Biochemistry;

   Sammanfattning : DNA polymerase epsilon (Pol ε) is a multi-subunit B-family DNA polymerase that is involved in leading strand DNA replication in eukaryotes. DNA Pol ε in yeast consists of four subunits, Pol2, Dpb2, Dpb3, and Dpb4. Pol2 is the catalytic subunit and Dpb2, Dpb3, and Dpb4 are the accessory subunits. LÄS MER

3. 2. Method development for the analysis of protein interactions

   Författare :Despoina Kermpatsou; Ola Söderberg; Maréne Landström; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; in situ; proximity ligation assay PLA ; strand displacement; proximity-dependent initiation of hybridisation chain reaction proxHCR ; protein complexes; rolling circle amplification RCA ; MolBoolean; vitamin D; 1; 25D3-MARRS receptor; PDIA3; ERp57; CYP24A1; c-Myc; prostate cancer; Molekylär cellbiologi; Molecular Cellbiology; Molekylär medicin; Molecular Medicine;

   Sammanfattning : Biological processes take place through interactions between macromolecules, such as nucleic acids and proteins. It is, therefore, fundamental to understand the functions of proteins and how they form complexes in order to carry out their role. LÄS MER

4. 3. Biochemical analysis of Pfh1, the essential Pif1 family helicase in Schizosaccharomyces pombe

   Författare :Jani B. Mohammad; Nasim Sabouri; Erik Johansson; Per Elias; Umeå universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; G4; Pfh1; Genome integrity; helicase; medicinsk biokemi; Medical Biochemistry;

   Sammanfattning : DNA stores the genetic information of all living organisms, and this information needs to be copied accurately and passed on to each daughter cell when a cell divides. However, the DNA replication machinery often meets obstacles in the genome that cause fork pausing and might result in DNA damage. LÄS MER

5. 4. Targeting double-stranded DNA with locked nucleic acid-based (LNA) oligonucleotides (ON) and DNA-binding peptide conjugates

   Författare :Y Vladimir Pabón-Martínez; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :;

   Sammanfattning : In this thesis we have studied two different types of molecules that bind to the major groove of the DNA: (i) oligonucleotides (ONs) containing locked nucleic acid (LNA) and in combination with intercalating agents (Twisted Intercalating Nucleic Acid, TINA, and Benzoquinoquinoxaline, BQQ) and (ii) peptides mimicking the leucine zipper transcription factor (GCN4 bZIP TF). Both ONs and peptides bind to the DNA double helix aiming to serve as transcription regulators of disease related genes. LÄS MER

7. 5. Regulation of Human Mitochondrial DNA Replication and Transcription

   Författare :Majda Mehmedovic; Göteborgs universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Mitochondria; mtDNA; replication; transcription; TFAM;

   Sammanfattning : Mitochondria are organelles in eukaryotic cells, which through oxidative phosphorylation (OXPHOS) produce most of the ATP used to drive cellular processes. The organelle contains its own genetic material, mitochondrial DNA (mtDNA), which encodes 13 key components of the OXPHOS machinery. LÄS MER