Sökning: "Strand displacement"

Visar resultat 1 - 5 av 8 avhandlingar innehållade orden Strand displacement.

  1. 1. Structural and biochemical basis for the high fidelity and processivity of DNA polymerase ε

    Detta är en avhandling från Umeå : Umeå universitet

    Författare :Rais Ahmad Ganai; Umeå universitet.; [2015]
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; DNA polymerase ε; Crystal structure; P domain; Pre-steady state kinetics; β-hairpin loop; Primer switching; Strand displacement; biokemi; Biochemistry;

    Sammanfattning : DNA polymerase epsilon (Pol ε) is a multi-subunit B-family DNA polymerase that is involved in leading strand DNA replication in eukaryotes. DNA Pol ε in yeast consists of four subunits, Pol2, Dpb2, Dpb3, and Dpb4. Pol2 is the catalytic subunit and Dpb2, Dpb3, and Dpb4 are the accessory subunits. LÄS MER

  2. 2. Biochemical analysis of Pfh1, the essential Pif1 family helicase in Schizosaccharomyces pombe

    Detta är en avhandling från Umeå : Umeå Universitet

    Författare :Jani B. Mohammad; Umeå universitet.; [2019]
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; G4; Pfh1; Genome integrity; helicase; medicinsk biokemi; Medical Biochemistry;

    Sammanfattning : DNA stores the genetic information of all living organisms, and this information needs to be copied accurately and passed on to each daughter cell when a cell divides. However, the DNA replication machinery often meets obstacles in the genome that cause fork pausing and might result in DNA damage. LÄS MER

  3. 3. Targeting double-stranded DNA with locked nucleic acid-based (LNA) oligonucleotides (ON) and DNA-binding peptide conjugates

    Detta är en avhandling från Stockholm : Karolinska Institutet, Dept of Laboratory Medicine

    Författare :Y. Vladimir Pabón-Martínez; Karolinska Institutet.; Karolinska Institutet.; [2016]
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES;

    Sammanfattning : In this thesis we have studied two different types of molecules that bind to the major groove of the DNA: (i) oligonucleotides (ONs) containing locked nucleic acid (LNA) and in combination with intercalating agents (Twisted Intercalating Nucleic Acid, TINA, and Benzoquinoquinoxaline, BQQ) and (ii) peptides mimicking the leucine zipper transcription factor (GCN4 bZIP TF). Both ONs and peptides bind to the DNA double helix aiming to serve as transcription regulators of disease related genes. LÄS MER

  4. 4. The interface of mitochondrial DNA transcription and replication

    Detta är en avhandling från Stockholm : Karolinska Institutet, Dept of Laboratory Medicine

    Författare :Paulina Hannele Wanrooij; Karolinska Institutet.; Karolinska Institutet.; [2012]
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES;

    Sammanfattning : Mitochondria are a dynamic network of subcellular organelles that produce the majority of cellular ATP through the process of oxidative phosphorylation (OXPHOS). The components of the respiratory chain are encoded by two separate genomes, nuclear DNA and mitochondrial DNA (mtDNA), and the proper maintenance of both of these genomic entities is therefore crucial for cellular ATP levels and the survival of the cell. LÄS MER

  5. 5. Padlock Probes and Rolling Circle Amplification New Possibilities for Sensitive Gene Detection

    Detta är en avhandling från Uppsala : Acta Universitatis Upsaliensis

    Författare :Maritha Mendel-Hartvig; Uppsala universitet.; [2002]
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; Padlock probes; RCR; in situ hybridization; branched DNA; Genetik; MEDICINE Dermatology and venerology; clinical genetics; internal medicine Clinical genetics; MEDICIN Dermatologi och venerologi; klinisk genetik; invärtesmedicin Klinisk genetik; Medical Genetics; medicinsk genetik;

    Sammanfattning : A series of novel methods for detection of known sequence variants in DNA, in particular single nucleotide polymorphism, using padlock probes and rolling circle replication are presented. DNA probes that can be circularized – padlock probes – are ideal for rolling circle replication. LÄS MER