Sökning: "Sortase A"

Visar resultat 1 - 5 av 9 avhandlingar innehållade orden Sortase A.

  1. 1. Towards Anti-Virulence Antimicrobials, Discovery and Development of Sortase A Inhibitors and Investigations of Bacterial Phenotypes

    Författare :Patrick M. Wehrli; Göteborgs universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Sortase A; SrtA; Inhibitors; Anti-virulence; Bacterial analysis; FTIR spectroscopy; Bacterial phenotyping; Design of Experiment; Multivariate Data Analysis; PCA; ToF-SIMS; Time-of-flight secondary-ion-mass-spectrometry; Lipid analysis; ppGpp; Stringent response;

    Sammanfattning : Antibiotic resistance is an emerging and serious threat to public health. Immediate actions are required to preserve current antibiotics while intensifying research efforts towards the development of new effective therapeutics. LÄS MER

  3. 2. Anti-Virulence Strategy Targeting Sortase A, A Structural Investigation of the Sortase A Enzyme, and the Identification, Synthesis, and Evaluation of Sortase A Inhibitors

    Författare :Ivana Uzelac; Göteborgs universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Kemi;

    Sammanfattning : The emergence of multi-resistant bacteria and their continuous spread is one of the greatest challenges when treating bacterial infections. Increased understanding of bacterial pathogenesis has revealed new strategies for treating bacteria-mediated diseases. LÄS MER

  4. 3. Combinatorial Protein Engineering Of Affibody Molecules Using E. Coli Display And Rational Design Of Affibody-Based Tracers For Medical Imaging

    Författare :Ken G. Andersson; John Löfblom; Stefan Ståhl; Harald Kolmar; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; directed evolution; microbial display; E. coli; Affibody molecule; autotransporter; medical imaging; HER receptor family; Sortase A; Bioteknologi; Biotechnology;

    Sammanfattning : Directed evolution is today an established strategy for generation of new affinity proteins. This thesis describes the development of a cell-display method using Escherichia coli for directed evolution of Affibody molecules. LÄS MER

  5. 4. Technological Advancements in Affinity Proteomics : From planar antibody microarrays towards a solution-based platform

    Författare :Mattias Brofelth; Institutionen för immunteknologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Affinity proteomics; Antibody microarrays; scFv; Biomarkers; MIAS;

    Sammanfattning : Abstract: Proteomics has the potential to deliver disease-associated biomarkers that could provide diagnostic, prognostic and predictive information to enable precision medicine. Affinity proteomics, most commonly based on antibodies and their ability to specifically capture target proteins, has emerged has a valuable tool in biomarker discovery. LÄS MER

  7. 5. Investigations of chemical and enzymatic functionalization of affinity proteins

    Författare :Anders Myrhammar; Amelie Eriksson Karlström; Per-Åke Nygren; Christian Heinis; KTH; []
    Nyckelord :Affinity protein; affibody; conjugation; solid phase peptide synthesis; proteolytic stability; peptide nucleic acid PNA .; Bioteknologi; Biotechnology;

    Sammanfattning : AbstractAffinity proteins are important reagents in research, diagnostics and therapeutic settings. The focus of this thesis has been on investigating different chemical and enzymatic strategies for engineering of affinity proteins to generate affinity reagents with improved or changed functionality. LÄS MER