Sökning: "Single-molecule"

Visar resultat 21 - 25 av 142 avhandlingar innehållade ordet Single-molecule.

  1. 21. Readout Strategies for Biomolecular Analyses

    Författare :Jenny Göransson; Mats Nilsson; Charles Cantor; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Padlock probe; Selector probe; Rolling circle amplification; Single molecule detection; Amplified single molecule; Random array; Molecular medicine genetics and pathology ; Molekylär medicin genetik och patologi ;

    Sammanfattning : This thesis describes three readout formats for molecular analyses. A common feature in all works is probing techniques that upon specific target recognition ideally results in equimolar amounts of DNA circles. These are then specifically amplified and detected using any of the techniques presented herein. LÄS MER

  3. 22. Development of novel bioanalytical assays with single-molecule readout for biomarker detection and drug candidate characterization

    Författare :Olov Wahlsten; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; QCM-D; biomarker detection; TIRF microscopy.; Bioanalytical assay; spectrofluorometry; fluorescence; single-molecule readout; drug discovery; liposomes;

    Sammanfattning : Bioanalytical assays with single-molecule readout for studying molecular interactions have in the past decades received increasing attention. The high sensitivity often offered by this readout scheme has for example enabled ultra-sensitive analyte detection, having important implications for monitoring early disease progression and the effects of drug treatment. LÄS MER

  4. 23. From single-cell transcriptomics to single-molecule counting

    Författare :Saiful Islam; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : RNA-sequencing (RNA-seq) technology has been progressing so fast in the last few years and made it possible to perform transcriptome analysis at single-cell level that was even unimaginable a few years before. Nowadays, the importance of gene expression analysis at the single-cell level is increasingly appreciated for the study of complex heterogeneous tissue. LÄS MER

  5. 24. Light polarization in single molecule spectroscopy: From visualization of structural anisotropy and energy transfer to super-resolution imaging with orientational contrast

    Författare :Daniel Thomsson; Kemisk fysik; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Single-molecule spectroscopy; π-conjugated polymers; blinking; fluorescence brightness; low temperature; polarization anisotropy; 2D polarization; energy transfer; super resolution; Fourier transform;

    Sammanfattning : Π-conjugated polymers are an important class of materials which can be used in e.g. photoelectric (solar cells) or electroluminescent (displays) devices. These materials are implemented in a number of applications these days since a lot is already known about them. LÄS MER

  7. 25. Development of Electrical Readouts for Amplified Single Molecule Detection

    Författare :Camilla Russell; Mats Nilsson; Björn Högberg; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Rolling circle amplification; metallization; electrical DNA detection; magnetic nanoparticles; dielectrophoresis; boranephosphonate modified nucleotides; DNA elongation; Biokemi; Biochemistry;

    Sammanfattning : Molecular diagnostics is a fast growing field with new technologies being developed constantly. There is a demand for more sophisticated molecular tools able to detect a multitude of molecules on a single molecule level with high specificity, able to distinguish them from other similar molecules. LÄS MER