Sökning: "Single cell genomics"

Visar resultat 1 - 5 av 41 avhandlingar innehållade orden Single cell genomics.

1. 1. Exploring human variations by droplet barcoding

   Författare :Pontus Höjer; Afshin Ahmadian; Pelin Sahlén; Lars Feuk; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; droplets; linked-read sequencing; DNA barcoding; proteomics; genomics; single cell; single extracellular vesicle; single exosome; pipelines; Biotechnology; Bioteknologi;

   Sammanfattning : Biological variations are being explored at ever-increasing rates through the rapid advancement of analytical techniques. Techniques like massively parallel sequencing empower scientists to accurately differentiate individuals’ genetic compositions, cellular functionalities, and healthy tissue from diseased. LÄS MER

3. 2. Computational Single-Cell Genomics Methods for Cell State Estimation and their Application to Hematopoietic Systems

   Författare :Parashar Dhapola; Avdelningen för molekylär hematologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Hematopoietic Stem Cell; Single-Cell; Genomics; Epigenomics; transcriptomics; bioinformatics;

   Sammanfattning : The focus of this doctoral dissertation was to develop novel and scalable computational genomics methods for analysing single-cell genomic modalities. Scarf, a highly memory-efficient single-cell data analysis toolkit, was developed to enable an analysis of very large-scale datasets on personal computers, and the execution of parallel workflows on server-scale computational systems. LÄS MER

4. 3. Uncovering the genetics underlying host-parasite interactions during Plasmodium falciparum malaria transmission

   Författare :Mubasher Mohammed; Johan Ankarklev; David Serre; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Malaria; Plasmodium falciparum; Genomics; single-cell RNA-seq; Parasite cell fate; Anopheles gambiae; ApiAP2 transcription factors; driver genes; computational biology; molekylär biovetenskap; Molecular Bioscience;

   Sammanfattning : In eukaryotes, cellular differentiation is often orchestrated by programmed arrays of activation and repression of genes underlying the specific phenotypes of cell-types. To complete its life cycle, the single-celled Apicomplexan parasite Plasmodium falciparum, the most deadly of the human malaria parasites, must repeatedly differentiate and convert into unique cell types that can exploit niches within their human and mosquito hosts. LÄS MER

5. 4. Into the Single-Verse : Subtle gene expression differences between virtually identical single cells are informative of gene regulation

   Författare :Marcel Tarbier; Marc Friedländer; Nils Blüthgen; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Single-cell; quantitative; RNA biology; miRNA; functional genomics; molekylär biovetenskap; Molecular Bioscience;

   Sammanfattning : The ability to profile transcriptomes and proteomes in a high-throughput fashion in single cells has truly revolutionized functional genomics, and countless functional and regulatory insights have been based on these technologies. While major applications include the discovery of new cell types and the a posteriori sorting of cell populations, studies of gene expression noise and gene co-expression have made use of this inter-cellular heterogeneity in a genuine quantitative fashion. LÄS MER

7. 5. Variational methods for phylogeny and single-cell genomics

   Författare :Hazal Koptagel; Jens Lagergren; Alexandre Bouchard-Côté; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Variational inference; single-cell DNA sequencing; cell lineage tree; Bayesian phylogenetic tree inference; Variationsinferens; encellig DNA-sekvensering; cellhärkomstträd; Bayesiansk fylogenetisk trädinferens; Varyasyonel çıkarım; tek hücre DNA dizilimi; hücre soy ağacı; Bayesçi filogenetik ağaç çıkarımı; Datalogi; Computer Science;

   Sammanfattning : The investigation of the evolutionary history of organisms, both at the cellular level and at the species level, is a relevant research topic in computational biology. These investigations lead to a deeper understanding of developmental history, cancer progression, the genetic similarity of species, and more. LÄS MER