Sökning: "Shotgun proteomics"

Visar resultat 1 - 5 av 22 avhandlingar innehållade orden Shotgun proteomics.

  1. 1. Proteomics in biomarker research : Insights into the effects of aging and environment on biological systems

    Författare :Hanna Amelina; Susana Cristobal; Jean-Charles Sanchez; Stockholms universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; NATURAL SCIENCES; proteomics; biomarker; peroxisomes; 2-dimensional gel electrophoresis 2-DE ; mass spectrometry MS ; aging; marine pollution; Biochemistry; Biokemi; Environmental toxicology; Miljötoxikologi; Cell and molecular biology; Cell- och molekylärbiologi; biokemi; Biochemistry;

    Sammanfattning : Proteomics is the global analysis of proteins that covers a broad range of technologies aimed at determining the identity and quantity of proteins expressed in the cell, their three-dimensional structure and interaction partners. In contrast to genome, proteome reflects more accurately on the dynamic state of the cell, tissue, or an organism. LÄS MER

  2. 2. The accuracy of statistical confidence estimates in shotgun proteomics

    Författare :Viktor Granholm; Lukas Käll; Alexey Nesvizhskii; Stockholms universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Proteomics; Peptides; Statistics; Mass spectrometry; Tandem mass spectrometry; Biochemistry towards Bioinformatics; biokemi med inriktning mot bioinformatik;

    Sammanfattning : High-throughput techniques are currently some of the most promising methods to study molecular biology, with the potential to improve medicine and enable new biological applications. In proteomics, the large scale study of proteins, the leading method is mass spectrometry. LÄS MER

  3. 3. The Heterocysts of Nostoc punctiforme : From Proteomics to Energy Transfer

    Författare :Tanai Cardona; Ann Magnuson; Matthias Rögner; Uppsala universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Nostoc punctiforme; heterocyst; photosynthesis; thylakoid; isolation; proteomics; photosystem; energy transfer; hydrogen; Biochemistry; Biokemi; biokemi; Biochemistry;

    Sammanfattning : The aim of this thesis is to provide a thorough characterization of the photosynthetic machinery from the heterocysts of Nostoc punctiforme strain ATCC 29133. In this thesis I describe the protocols I have optimized for the isolation of thylakoids from vegetative cells, the purification of heterocysts and the isolation of thylakoids from the purified heterocysts. LÄS MER

  4. 4. Parameterized algorithms for hitting set variants applied to protein identification in shotgun proteomics

    Författare :Leonid Molokov; Chalmers University of Technology; []
    Nyckelord :NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; NATURAL SCIENCES; Union Editing; Fixed Parameter Tractable; Shotgun proteomics; Hitting Set; Parameterized complexity; Hypergraph; Set Cover;

    Sammanfattning : This work is dedicated to problem of protein identification in bottom-up proteomics, and in particular, in shotgun proteomics. It is aimed on providing a new way of interpretation of peak lists. LÄS MER

  5. 5. Quality Control for high-throughput Quantitative Proteomics - Harnessing the potential of label-free LC-MS

    Författare :Marianne Sandin; Institutionen för Immunteknologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; proteomics; mass spectrometry; quality control; data analysis; label-free; feature detection; alignment; biomarker discovery; separation techniques;

    Sammanfattning : Multiplex protein quantification, proteomics, is essential for uncovering new biomarkers and understanding biology. Liquid chromatography coupled to mass spectrometry (LC-MS) is the predominant technique for these measurements. LÄS MER