Sökning: "Shotgun proteomics"
Visar resultat 1 - 5 av 22 avhandlingar innehållade orden Shotgun proteomics.
1. Proteomics in biomarker research : Insights into the effects of aging and environment on biological systems
Sammanfattning : Proteomics is the global analysis of proteins that covers a broad range of technologies aimed at determining the identity and quantity of proteins expressed in the cell, their three-dimensional structure and interaction partners. In contrast to genome, proteome reflects more accurately on the dynamic state of the cell, tissue, or an organism. LÄS MER
Sammanfattning : High-throughput techniques are currently some of the most promising methods to study molecular biology, with the potential to improve medicine and enable new biological applications. In proteomics, the large scale study of proteins, the leading method is mass spectrometry. LÄS MER
Sammanfattning : The aim of this thesis is to provide a thorough characterization of the photosynthetic machinery from the heterocysts of Nostoc punctiforme strain ATCC 29133. In this thesis I describe the protocols I have optimized for the isolation of thylakoids from vegetative cells, the purification of heterocysts and the isolation of thylakoids from the purified heterocysts. LÄS MER
4. Parameterized algorithms for hitting set variants applied to protein identification in shotgun proteomics
Sammanfattning : This work is dedicated to problem of protein identification in bottom-up proteomics, and in particular, in shotgun proteomics. It is aimed on providing a new way of interpretation of peak lists. LÄS MER
5. Quality Control for high-throughput Quantitative Proteomics - Harnessing the potential of label-free LC-MS
Sammanfattning : Multiplex protein quantification, proteomics, is essential for uncovering new biomarkers and understanding biology. Liquid chromatography coupled to mass spectrometry (LC-MS) is the predominant technique for these measurements. LÄS MER