Sökning: "Shine-Dalgarno sequence"

Visar resultat 1 - 5 av 6 avhandlingar innehållade orden Shine-Dalgarno sequence.

  1. 1. Studies on translation initiation and gene expression in Escherichia coli

    Författare :Ernesto I. Gonzalez de Valdivia; Leif Isaksson; Stephen Douthwaite; Stockholms universitet; []
    Nyckelord :Downstream region; NGG codons; abortive translation; drop-off; Shine-Dalgarno sequence; gene expression and Escherichia coli; NATURAL SCIENCES; NATURVETENSKAP;

    Sammanfattning : In prokaryotes, several mRNA sequences surrounding the initiation codon have been found to influence the translation process; these include the downstream region and its codon context, the Shine-Dalgarno sequence and the S1 ribosomal protein-binding site. In this thesis, the purpose has been to study the role of the downstream region and Shine-Dalgarno-like sequences on early translation elongation and gene expression in Escherichia coli. LÄS MER

  3. 2. Ribosome standby sites and other structural aspects of translation initiation regions in Escherichia coli

    Författare :Maaike M. Sterk; Gerhart E. H. Wagner; Fredrik Söderbom; Isabella Moll; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Ribosome standby sites; translation initiation; mRNA structure; bacteria;

    Sammanfattning : Translation initiation, which is rate-limiting in protein synthesis, is often the step at which regulation occurs. Here, we investigated several mechanisms of translation initiation in Escherichia coli, including their control. LÄS MER

  4. 3. Structural and Genetic Studies of Translation in Escherichia coli

    Författare :Qing Zhao; Leif Isaksson; Lars-Göran Öfverstedt; Odd Nygård; Stockholms universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; electron tomography; ribosome; 30S subunit; 50S subunit; rifampicin; Escherichia coli; Translation initiation; Initiation codon; Downstream Region DR ; Microbiology; immunology; infectious diseases; Mikrobiologi; immunologi; infektionssjukdomar;

    Sammanfattning : Ribosomes are the universal ribonucleoprotein organelles that translate the genetic message from mRNA to protein. In prokaryotes, the ribosomal subunits are 30S and 50S subunit, which bind together during the translation process forming 70S ribosome. LÄS MER

  5. 4. Studies on Translation Initiation and trans-Translation

    Författare :Serhiy Surkov; Leif Isaksson; Udo Bläsi; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; E. coli; ribosome; IF1; tmRNA; trans-translaton; Molecular biology; Molekylärbiologi; molekylärgenetik; Molecular Genetics;

    Sammanfattning :  Translation initiation factor 1 (IF1) is an essential bacterial protein, without any clearly defined function in translation initiation. Same point mutants in this protein exhibit cold-sensitivity. In addition, a sequence specific increase in test protein expression has been observed for these mutants. LÄS MER

  7. 5. The Involvement of the structures of antisense RNA, target RNA, and their complexes in plasmid R1 copy number regulation

    Författare :Charlotta Ingvoldstad; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Cell and molecular biology; Cell- och molekylärbiologi; Cell and molecular biology; Cell- och molekylärbiologi; mikrobiologi; Microbiology;

    Sammanfattning : Plasmid R1 is a low-copy-number plasmid which belongs to the incompatibility group FII. R1 regulates its own copy number. Initiation of replication of plasmid R1 requires the binding of the RepA protein to oriR1. RepA acts in cis and has to be synthesized de novo for each round of replication. LÄS MER