Sökning: "Sf9 cells"

Visar resultat 1 - 5 av 6 avhandlingar innehållade orden Sf9 cells.

1. 1. Regulation of productivity in Trichoplusia ni and Spodoptera frugiperda Sf9 serum-free cultures

   Författare :Karin Calles; Lena Häggström; Gunnel Dalhammar; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biotechnology; Insect cells; BEVS; Spodoptera frugiperda; Sf9; Trichoplusia ni; BTI-Tn-5B1-4; specific productivity; conditioned medium; conditioned medium factors; cell cycle; synchronization; G2 M; Bioteknik; Bioengineering; Bioteknik;

   Sammanfattning : The aim of this work has been to characterize the effects of conditioned medium (CM) on insect cell productivity and physiology in order to get a better understanding about the mechanisms that regulate productivity in serum-free media. Two cell lines have been investigated, Spodoptera frugiperda (Sf9) and Trichoplusia ni (T. ni, BTI-Tn-5B1-4). LÄS MER

3. 2. Physiological effects of conditioned medium and passage number on Spodoptera frugiperda Sf9 serum free cultures

   Författare :Ingrid Svensson; Lena Häggström; Mats Martinelle; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biotechnology; Spodoptera frugiperda Sf9; insect cell cultures; conditioned medium; cell cycle phase dynamics; proteolytic activity; antibacterial activity; recombinant protein production; Bioteknik; Bioengineering; Bioteknik;

   Sammanfattning : The aim of this study was to better understand the role of conditioned medium (CM) in Spodoptera frugiperda Sf9 insect cell proliferation and recombinant protein production using the baculovirus expression system. CM was found to stimulate cell proliferation. LÄS MER

4. 3. Physiology and productivity of serum-free Spodoptera frugiperda Sf9 insect cell cultures

   Författare :Eva Lindskog; Lena Häggström; Spiros N. Agathos; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Animalcellteknologi; Bioengineering; Bioteknik;

   Sammanfattning : The objective of this study was to investigate the mechanisms and factors controlling growth and proliferation in serum-free Spodoptera frugiperda Sf9 cultures as well as the implications of these factors for protein production in the baculovirus expression system. The physiology of recently thawed, low passage (Lp) Sf9 cultures, were compared to high passage (Hp) cultures at p>100. LÄS MER

5. 4. Expression and post-translational modification of purple acid phosphatase in insect and mammalian cells

   Författare :Yunling Wang; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :;

   Sammanfattning : Purple acid phosphatase (PAP) is an acidic metallohydrolase that contain a binuclear iron center in its active site. In mammals, this enzyme is also referred to as tartrate-resistant acid phosphatase (TRAPs), uteroferrin or type 5 acid phosphatase. LÄS MER

7. 5. Studies on O-glycosylation of mucin-type proteins and their binding to antibodies, bacterial toxins and viral receptors

   Författare :Stefan Gaunitz; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :;

   Sammanfattning : Carbohydrates are ubiquitous on the surface of all cells in mammals where they are involved in interactions with the surroundings (extracellular matrix), other cells (including self and non-self) and microbes (bacteria and virus). Carbohydrate-protein interactions in nature are often mediated via multivalent binding where the combined strength of multiple receptor- ligand interactions results in a binding that is highly specific and strong. LÄS MER