Sökning: "Sample Preparation"

Visar resultat 21 - 25 av 335 avhandlingar innehållade orden Sample Preparation.

  1. 21. Microfluidic electrocapture in proteomic sample preparation for mass spectrometry

    Författare :Susanne Vollmer; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Proteomics is a technology-driven discipline and very labor-intensive. Over the last couple of years, methods with microfluidic systems have been used to simplify analytical procedures via integration of laboratory processes and equipment into miniaturized formats to achieve reproducible and high throughput analyses of biomolecules. LÄS MER

  3. 22. PCR-based detection of microorganisms in complex biological samples

    Författare :Pär-G Lantz; Teknisk mikrobiologi; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Density gradient centrifugation; DNA extraction; Aqueous two-phase systems; Meat; Faeces; Cheese; Yeast; Helicobacter pylori; Yersinia enterocolitica; Listeria monocytogenes; PCR; Inhibition; Sample preparation; Microbiology; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi;

    Sammanfattning : The detection of microorganisms by PCR can be divided into four steps: (1) sample collection, (2) sample preparation, (3) DNA amplification and (4) detection of PCR products. The major problem in developing PCR-based detection methods is the sample preparation step. LÄS MER

  4. 23. Development of Methods for Determination of Fat and Trace Constituents in Food, with Emphasis on Supercritical Fluid Extraction

    Författare :Hans Berg; Centrum för analys och syntes; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Supercritical fluid extraction; Steroids; SFE; Sample preparation; Meat; Lipid classes; Food; Fatty acid composition; Fat content; 3-butanediol; 2; Diacetyl; Acetoin; Androstenone; vitamins A and E.; Analytical chemistry; Analytisk kemi;

    Sammanfattning : Sample preparation is the most time-consuming step in an analysis. Traditional sample preparation techniques consume large amounts of organic solvents, are time-consuming and are difficult to automate. LÄS MER

  5. 24. Non-porous membrane extractions in contemporary analysis

    Författare :Jan Norberg; Centrum för analys och syntes; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; extracting syringe; micro-porous membrane extraction; supported liquid membrane; solvent free; selectivity; clean-up; enrichment; Sample preparation; automation; Analytical chemistry; Analytisk kemi;

    Sammanfattning : To meet the desirable characteristic for sample preparation in contemporary, i. e. automation, high enrichment, clean-up, selectivity and essential solvent free techniques, the use of non-porous membrane extraction techniques is addressed. LÄS MER

  7. 25. High-throughput screening of solid-phase extraction materials using mass spectrometry

    Författare :Kishore Jagadeesan; Avdelningen för Biomedicinsk teknik; []
    Nyckelord :mass spectromery; protein separation; sample preparation; Molecularly imprinted polymer; Data analysis; Data visualization; Miniaturisation; Proteomics;

    Sammanfattning : In biomarker analysis, sample preparation plays a crucial step in order to extract, isolate and concentrate the analytes of interest. Among the various sample preparation techniques, solid phase extraction (SPE) is one of the most common and popular, due to the high enrichment factor, good recovery, low consumption of organic solvents and the possibility to automate (off- or on-line) the whole process. LÄS MER