Sökning: "SINGLE-STRANDED-DNA"

Visar resultat 1 - 5 av 28 avhandlingar innehållade ordet SINGLE-STRANDED-DNA.

1. 1. Single-stranded DNA : methods and application in nanotechnology

   Författare :Giulio Bernardinelli; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :;

   Sammanfattning : Basic molecular and cell research, production of recombinant proteins, diagnostic detection of genetic mutations, construction of nanostructures and high-throughput DNA sequencing are only a few examples of the diverse set of applications that are amenable thanks to the availability of synthetic DNA polymers in biomedicine. Strategic investments and technical progress together with the introduction of automation in the synthesis of DNA oligomers enabled to transform, in just a few decades, a process mastered only by a niche of biochemists into an affordable and available custom-made product to every scientific field. LÄS MER

3. 2. Method development and applications of Pyrosequencing technology

   Författare :Baback Gharizadeh; KTH; []
   Nyckelord :apyrase; bacterial identification; dATP?S; EST sequencing; fungal identification; human papillomavirus HPV ; microbial and viral typing; multiple sequencing primer method; Pyrosequencing technology; Sequenase; single-stranded DNA-binding protein SS;

   Sammanfattning : The ability to determine nucleic acid sequences is one ofthe most important platforms for the detailed study ofbiological systems. Pyrosequencing technology is a relativelynovel DNA sequencing technique with multifaceted uniquecharacteristics, adjustable to different strategies, formatsand instrumentations. LÄS MER

4. 3. Combinatorial Methodology. Screening for affinity specific ligands to biological and artificial receptors

   Författare :Margareta Krook; Tillämpad biokemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; peptide library; combinatorial; screening; phage display; affinity; DNA-interaction; chymotrypsin; peptide inhibitor; antibody; IgG; Fc; molecular imprinting; steroid; receptor; Biochemistry; Metabolism; Biokemi; Biotechnology; Bioteknik;

   Sammanfattning : Combinatorial screening methodology has been employed to select new affinity specific ligands to bilogical and artificial receptors. The screening methodology, in particular screening of combinatorial phage display peptide libraries against target molecules differing in function and shape, is focused upon. LÄS MER

5. 4. DNA precursor biosynthesis-allosteric regulation and medical applications

   Författare :Reza Rofougaran; Anders Hofer; Thomas Borén; Staffan Eriksson; Umeå universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; DNA biosynthesis; ribonucleotide reductase; allosteric regulation; Trypanosoma brucei; adenosine kinase; nucleoside analogs; mitochondrial DNA; TWINKLE; Biokemi; medicinsk biokemi; Medical Biochemistry;

   Sammanfattning : Ribonucleotide reductase (RNR) is a key enzyme for de novo dNTP biosynthesis. We have studied nucleotide-dependent oligomerization of the allosterically regulated mammalian RNR using a mass spectrometry–related technique called Gas-phase Electrophoretic Mobility Macromolecule Analysis (GEMMA). LÄS MER

7. 5. Development of an Ultrasensitive Capacitive DNA-sensor: A promising tool towards microbial diagnostics

   Författare :Ally Mahadhy; Bioteknik; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Capacitive DNA-sensor; Enterobacteriaceae; mecA gene; Methicillin resistance; automated sequential injection analysis; sandwichhybridization; elevated temperature; polytyramine;

   Sammanfattning : Fast and sensitive detection of pathogenic microbial cells is a highly important task in medical diagnostics, environmental analysis and evaluation of food safety. Accordingly, the idea of microorganism identification by the recognition of specific DNA sequence using electrochemical technique is one of the leading researches in the development of diagnostic devices. LÄS MER