Sökning: "S-adenosyl-methionine"

Visar resultat 1 - 5 av 11 avhandlingar innehållade ordet S-adenosyl-methionine.

  1. 1. Antibiotic Resistance and the Cellular Currency S-adenosyl-methionine : Modification of aminoglycosides and nucleic acids

    Författare :Sandesh Kanchugal P; Maria Selmer; Ditlev Brodersen; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Antibiotic resistance; streptomycin; spectinomycin; X-ray crystallography; methyltransferase; Biology with specialization in Structural Biology; Biologi med inriktning mot strukturbiologi;

    Sammanfattning : Streptomycin and spectinomycin are antibiotics that bind to ribosomes and inhibit protein synthesis. Common resistance mechanisms involve enzymatic modification of the two drugs by aminoglycoside nucleotidyltransferases (ANTs). The first part of this thesis covers the structural mechanism of two ANT enzymes. LÄS MER

  3. 2. Ribosomal RNA Modification Enzymes : Structural and functional studies of two methyltransferases for 23S rRNA modification in Escherichia coli

    Författare :Avinash S. Punekar; Maria Selmer; Martino Bolognesi; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Escherichia coli; ribosome biogenesis; ribosome assembly; ribosomal RNA; peptidyltransferase center; domain V; post-transcriptional modification; methyltransferases; S-adenosyl-methionine; RlmM; Cm2498; RlmJ; m6A2030; X-ray crystallography; substrate recognition; substrate specificity; catalytic mechanism; evolution; Biology with specialization in Structural Biology; Biologi med inriktning mot strukturbiologi; Biokemi; Biochemistry;

    Sammanfattning : Escherichia coli ribosomal RNA (rRNA) is post-transcriptionally modified by site-specific enzymes. The role of most modifications is not known and little is known about how these enzymes recognize their target substrates. LÄS MER

  4. 3. The Importance of Being Promiscuous : Understanding enzyme function, specificity, and evolution through structure

    Författare :Annika Söderholm; Maria Selmer; Birte Höcker; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Enzyme evolution; enzyme promiscuity; X-ray crystallography.;

    Sammanfattning : Enzymes are known to be amazingly specific and efficient catalysts. However, many enzymes also have so-called promiscuous functions, i.e., they are able to catalyze other reactions than their main one. LÄS MER

  5. 4. To reduce a ribonucleotide – Radical solutions in enzymology in form and function

    Författare :Oskar Aurelius; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Ribonucleotide reductase; X-ray crystallography; Small-angle X-ray scattering; nucleotide metabolism; protein biophysics; metalloproteins; anaerobic enzymology;

    Sammanfattning : It has been more than 50 years since the enzyme system ribonucleotide reductase (RNR), catalysing the reduction of ribonucleotides to deoxyribonucleotides, was first discovered. RNR was also the first time that radical chemistry was revealed in an enzyme. LÄS MER

  7. 5. Meticulous control of the T3SS of Yersinia is essential for full virulence

    Författare :Ann-Catrin Björnfot; Hans Wolf-Watz; Kelly T. Hughes; Umeå universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Yersinia; type III secretion; YscU; substrate specificity switch; heat shock proteins; VagH; methyltransferase; virulence; Molecular biology; Molekylärbiologi; molekylärbiologi; Molecular Biology;

    Sammanfattning : The type III secretion system (T3SS) of pathogenic Yersinia pseudotuberculosis is involved in virulence. The syringe-like secretion system spans both bacterial membranes and is responsible for the ability of Yersinia to transfer toxic proteins (Yop proteins) into the eukaryotic target cell. LÄS MER