Sökning: "Reverse transcription"

Visar resultat 1 - 5 av 95 avhandlingar innehållade orden Reverse transcription.

  1. 1. Droplet Microfluidics reverse transcription and PCR towards Single Cell and Exosome Analysis

    Författare :Lovisa Söderberg; Helene Andersson Svahn; Håkan Jönsson; Valérie Taly; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Droplet microfluidics; Reverse transcription; Droplet PCR; High Throughput biology; Single cell Analysis; Exosomes; Bioteknologi; Biotechnology;

    Sammanfattning : Miniaturization of biological analysis is a trend in the field of biotechnology aiming to increase resolution and sensitivity in biological assays. Decreasing the reaction volumes to analyze fewer analytes in each reaction vessel enables the detection of rare analytes in a vast background of more common variants. LÄS MER

  3. 2. Human Endogenous Retroviruses: Expression and Evolutionary Relationships

    Författare :Marie-Louise Andersson; Avdelningen för medicinsk mikrobiologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Mikrobiologi; mycology; virology; bacteriology; Microbiology; bakteriologi; expression; transcription; reverse transcriptase; HERV; phylogeny; virologi; mykologi;

    Sammanfattning : The human genome contains genetic elements which are more or less similar to infectious retroviruses. These are called human endogenous retroviruses (HERVs) and are thought to be remnants of infections in the primate lineage. LÄS MER

  4. 3. Targeting HIV-1 entry and reverse transcription by vaccination

    Författare :Bartek Zuber; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :HIV-1; DNA vaccination; coreceptor; CCR5; RT; drug resistance; CpG; pseudovirus;

    Sammanfattning : Human immunodeficiency virus type I (HIV-1) is a complex retrovirus, which uses the CD4 receptor and chemokine receptors to infect its target cells. The chemokine receptor CCR5 is essential for primary HIV-1 infection. The hallmark of retroviruses is the enzyme reverse transcriptase (RT), which transcribes the virus genome from RNA to DNA. LÄS MER

  5. 4. Mediator and its role in non-coding RNA and chromatin regualtion

    Författare :Jonas O P Carlsten; Göteborgs universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Mediator; transcription; centromere; telomere; tRNA; exosome;

    Sammanfattning : Mediator is a multiprotein complex required for the regulation of RNA Polymerase II (Pol II) transcription. Mediator transmits regulatory signals from activators and repressors to the Pol II machinery at the promoter, but the complex has also many other functions related to control of gene transcription. LÄS MER

  7. 5. Gene expression profiling with real-time PCR

    Författare :Anders Ståhlberg; Chalmers tekniska högskola; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; single cell biology; reverse transcription; LightUp probe; cancer diagnostics; real-time PCR; gene expression profiling; tumor biology;

    Sammanfattning : The sequence of the entire human genome is available today, and this information can be used to understand, characterize, and treat complex diseases such as cancer. Many cellular functions related to survival, growth and differentiation are reflected in the gene expression pattern, and the ability to quantify transcription levels of specific genes is, and always has been, central to research on gene function. LÄS MER