Sökning: "Real-time PCR"

Visar resultat 11 - 15 av 296 avhandlingar innehållade orden Real-time PCR.

  1. 11. PCR inhibition mechanisms in forensic DNA analysis

    Författare :Maja Sidstedt; Teknisk mikrobiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; PCR inhibition mechanisms; Real-time PCR; Digital PCR; Massively parallel sequencing;

    Sammanfattning : In forensic DNA analysis, the polymerase chain reaction (PCR) enables DNAanalysis of minute biological crime scene traces. PCR is an enzymatic reaction for amplification of specific DNA fragments involving both biochemical andbiophysical processes. The main analytical challenge is posed by the nature of the samples of interest. LÄS MER

  3. 12. New specific and non-specific real-time PCR chemistries

    Författare :Ashraf Ahmad; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES;

    Sammanfattning : .... LÄS MER

  4. 13. Detection and Quantification of Variable Viral RNA by Real-Time PCR Assays

    Författare :Shaman Muradrasoli; Jonas Blomberg; Göran Friman; Maria Brytting; Uppsala universitet; []
    Nyckelord :Virology; Diagnosis of Viral RNA; QPCR; RNA virus; broadly targeted PCR; probe design strategy; Infectious diseases; Medicin; Medicine;

    Sammanfattning : As the area of nucleic acid based technologies develops, so will our understanding of how structural variations in DNA and RNA pathogens are associated with disease. The overall goal of this thesis is the development of broadly targeted measurement techniques for variable viral RNA by Real-Time PCR (here referred to as quantitative reverse transcriptase PCR, QRT-PCR). LÄS MER

  5. 14. Molecular Diagnosis of Common Viral Infectious Diseases Based on Real-Time PCR

    Författare :Nahla Mohamed; Jonas Blomberg; Jan Fohlman; Elgh Fredrik; Uppsala universitet; []
    Nyckelord :Molecular biology; Microbiology; Virology; rTth DNA polymerase; UNG; QPCR; RNA virus; broadly targeted PCR; Molekylärbiologi;

    Sammanfattning : Molecular biology has become an integral part of the diagnosis of infectious diseases. Recently, quantitative real-time PCR (QPCR) methods (often in the form of so-called TaqMan® systems) have been developed for the diagnosis of a wide range of infectious diseases; these techniques found valuable clinical application in the diagnosis and evaluation of progress and therapeutic success of viral diseases. LÄS MER

  7. 15. PCR detection of Streptococcus pneumoniae and Haemophilus influenzae in pneumonia patients

    Författare :Guma M. K. Abdeldaim; Björn Herrmann; Jonas Blomberg; Kristoffer Strålin; Birgitta Henriques; Uppsala universitet; []
    Nyckelord :Lower respiratory tract infections; Pneumonia; Streptococcus pneumoniae; Haemophilus influenzae; real-time PCR;

    Sammanfattning : PCR is a rapid, reproducible method for nucleic acid detection. However, this technology displays significant deficiencies when applied in clinical microbiology. This work’s aim was to improve current diagnostics and provide sensitive and quantitative real-time PCRs. LÄS MER