Sökning: "Real-Time Cell Binding Assays"

Visar resultat 1 - 5 av 9 avhandlingar innehållade orden Real-Time Cell Binding Assays.

1. 1. Novel Methods for Analysis of Heterogeneous Protein-Cell Interactions : Resolving How the Epidermal Growth Factor Binds to Its Receptor

   Författare :Hanna Björkelund; Karl Andersson; Lars Gedda; Yosef Yarden; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Heterogeneity; Kinetics; EGFR; HER2; LigandTracer; Interaction Map; Internalization; Specificity; Medical Science; Medicinsk vetenskap;

   Sammanfattning : Cells are complex biological units with advanced signalling systems, a dynamic capacity to adapt to its environment, and the ability to divide and grow. In fact, they are of such high level of complexity that it has deemed extremely difficult or even impossible to completely understand cells as complete units. LÄS MER

3. 2. Towards time-resolved molecular interaction assays in living bacteria

   Författare :João Crispim Encarnação; Karl Andersson; Marika Nestor; U. Helena Danielson; Hélène Munier-Lehmann; Uppsala universitet; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Intracellular Molecular Interactions; Real-Time Cell Binding Assays; Bacteria; LigandTracer; Kinetics; Biokemi; Biochemistry; Biofarmaci; Biopharmaceutics; Medicinsk vetenskap; Medical Science;

   Sammanfattning : Rare and neglected diseases such as multidrug resistant (MDR) tuberculosis, malaria and trypanosomiasis are re-emerging in Europe. New strategies are needed to accelerate drug discovery to fight these pathogens. LÄS MER

4. 3. Deciphering Binding Patterns of Therapeutic Antibodies with Immune Cells : From Method Development to Application

   Författare :Sina Bondza; Jos Buijs; Marika Nestor; Mark Cragg; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; affinity; binding kinetics; CD20; cell-based assay; immunology; receptor-ligand interaction; therapeutic antibody; Immunologi; Immunology; Medical Cell Biology; Medicinsk cellbiologi;

   Sammanfattning : Reversible binding, for example between signaling molecules and receptors on the cell surface, is one of the main means to communicate information in cellular systems. Knowledge about how molecules interact is crucial for both understanding biological function and for therapeutic intervention. LÄS MER

5. 4. The effect of chronic alcoholism on epigenetic patterns regulating gene expression and neurodegeneration in the human brain

   Författare :Sofia Johansson; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :Alcoholism; cognition; drug dependence; brain; frontal cortex; motor cortex; LUMA; epigenetics; methylation; gene expression; endogenous controls; real-time PCR.;

   Sammanfattning : Chronic alcoholism causes about 3.2% of all deaths worldwide. It has an association to about 60 different diseases and injuries with enormous social and economical impact. Despite these facts, very few pharmacological treatments exist and we can still not explain the mechanisms behind addiction, craving and cognitive impairments seen in the abuser. LÄS MER

7. 5. Accelerating the discovery of drugs for Neglected Tropical Diseases using biophysical methods

   Författare :Giulia Opassi; Helena Danielson; Jenny Sandmark; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Neglected Tropical Diseases; Drug Discovery; Biochemistry; Biophysics; Kinetic; Fragment Based Lead Discovery; Assay Development;

   Sammanfattning : Even though they account for 10% of the global disease burden and besides the new record of funding in 2018, tropical diseases are still neglected by the majority of pharmaceutical companies and public funding. The reason can be mostly found in the fact that for these diseases a conventional drug discovery process is often too expensive. LÄS MER