Sökning: "Real time RT-PCR"

Visar resultat 1 - 5 av 76 avhandlingar innehållade orden Real time RT-PCR.

  1. 1. Real-time PCR and its application for protein quantification

    Författare :Kristina Lind; Chalmers tekniska högskola; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; ELISA; real-time immuno-PCR; real-time PCR; melt curve analysis; PSA; 3’UTR; TAP-tag; BOXTO; immuno-qPCR;

    Sammanfattning : The ability to quantify specific DNA sequences with real-time PCR has increased the possibilities for sensitive DNA analysis enormously. This sensitive technique can in optimal conditions detect one DNA copy and has a large quantification range. LÄS MER

  3. 2. The Origin of the Genus Flavivirus and the Ecology of Tick-Borne Pathogens

    Författare :John H.-O. Pettersson; Thomas Jaenson; Sven Bergström; Kenneth Nilsson; Mikael Thollesson; Per-Eric Lindgren; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Flavivirus; Virus dating; Molecular dating; Biogeography; Ixodes ricinus; Minimum infection rate; TBE; Tick-borne encephalitis virus; Rickettsia helvetica; Anaplasma phagocytophilum; Infection prevalence; RT-PCR; Biology with specialization in Systematics; Biologi med inriktning mot systematik;

    Sammanfattning : The present thesis examines questions related to the temporal origin of the Flavivirus genus and the ecology of tick-borne pathogens. In the first study, we date the origin and divergence time of the Flavivirus genus. It has been argued that the first flaviviruses originated after the last glacial maximum. LÄS MER

  4. 3. High-resolution Studies of mRNA Expression in Brain : A Search for Genes Differently Expressed in Schizophrenia

    Författare :Anja Castensson; Elena Jazin; Terence Speed; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; mRNA; gene expression; real-time RT-PCR; schizophrenia; 5-HT serotonin receptor 2C; brain; psychiatric genetics; Genetik; Clinical genetics; Klinisk genetik;

    Sammanfattning : Gene expression differences between patients and controls can be used to find susceptibility genes and drug targets for a disease. High-resolution strategies are required because the differences between the investigated groups may be small and numerous factors may affect the mRNA quantity. LÄS MER

  5. 4. The Identification and Characterisation of LRIG Gene Family and Its Expression in Astrocytic Tumours

    Författare :Dongsheng Guo; Tomas Ekström; Umeå universitet; []
    Nyckelord :astrocytoma; brain; EGFR; LRIG; leucine-rich repeat; real-time RT-PCR; onkologi; Oncology;

    Sammanfattning : Gliomas are the most common primary brain tumours, and their capacity to invade surrounding normal brain prevents complete removal of the tumour. Malignant glioma has still a poor prognosis. However, with the rapid development of molecular biology our understanding about glioma has increased dramatically. LÄS MER

  7. 5. Effects of Biomechanical Stress on Gene Regulation in Vascular Cells

    Författare :Maria Carlström; Göteborgs universitet; []
    Nyckelord :shear stress; intraluminal pressure; endothelium; gene expression; DNA microarray; real-time RT-PCR; reference genes; smooth muscle cells; P2 receptors; TNF-alpha; urokinase-type plasminogen activator;

    Sammanfattning : The vascular vessel wall is constantly exposed to biomechanical forces, such as shear and tensile stress. Biomechanical forces are important for several physiological and pathological processes and have been shown to regulate a number of fundamental vascular functions, such as vascular tone and remodeling processes. LÄS MER