Sökning: "Real Time PCR"

Visar resultat 1 - 5 av 301 avhandlingar innehållade orden Real Time PCR.

  1. 1. Kinetics Quality Assessment for real-time PCR

    Författare :Tzachi Bar; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; kinetics quality assessment; PCR quality assessment; outlier detection; real-time PCR;

    Sammanfattning : For proper DNA quantification by real-time PCR, compared samples should have similar PCR efficiencies. This assumption might not be always correct, as suggested by hundreds of publications dealing with PCR inhibition. LÄS MER

  3. 2. Diagnostic PCR for the Detection of Yersinia enterocolitica and Salmonella in the Food-Chain: Reliability of PCR Performance

    Författare :Rickard Knutsson; Teknisk mikrobiologi; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Livsmedelsteknik; Food and drink technology; Kemiteknik och kemisk teknologi; Chemical technology and engineering; real-time PCR; YPCE medium; Enrichment PCR; DNA polymerases; PCR inhibitors; pre-PCR processing; Salmonella; Diagnostic PCR; Yersinia enterocolitica; Microbiology; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi;

    Sammanfattning : This thesis deals with the methodological advances of diagnostic PCR including reliability of PCR and pre-PCR processing of food and feed samples. Diagnostic PCR has been greatly improved by the introduction of the second generation of PCR, so-called real-time PCR. LÄS MER

  4. 3. Real-time PCR and its application for protein quantification

    Författare :Kristina Lind; Chalmers tekniska högskola; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; ELISA; real-time immuno-PCR; real-time PCR; melt curve analysis; PSA; 3’UTR; TAP-tag; BOXTO; immuno-qPCR;

    Sammanfattning : The ability to quantify specific DNA sequences with real-time PCR has increased the possibilities for sensitive DNA analysis enormously. This sensitive technique can in optimal conditions detect one DNA copy and has a large quantification range. LÄS MER

  5. 4. Kinetic Outlier Detection (KOD) in real-time PCR

    Författare :Tzachi Bar; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; PCR quality assessment; Real-time PCR; KOD; Kinetic Outliner Detection;

    Sammanfattning : .... LÄS MER

  7. 5. Gene expression profiling with real-time PCR

    Författare :Anders Ståhlberg; Chalmers tekniska högskola; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; single cell biology; reverse transcription; LightUp probe; cancer diagnostics; real-time PCR; gene expression profiling; tumor biology;

    Sammanfattning : The sequence of the entire human genome is available today, and this information can be used to understand, characterize, and treat complex diseases such as cancer. Many cellular functions related to survival, growth and differentiation are reflected in the gene expression pattern, and the ability to quantify transcription levels of specific genes is, and always has been, central to research on gene function. LÄS MER