Sökning: "RT-PCR"

Visar resultat 6 - 10 av 292 avhandlingar innehållade ordet RT-PCR.

  1. 6. Radiolabelled Oligonucleotides for Evaluation of in vivo Hybridisation Utilising PET Methodology

    Författare :Gábor Lendvai; Mats Bergström; Sergio Estrada; Barbro Eriksson; Raymond Josephsson; Bertrand Tavitian; Uppsala universitet; []
    Nyckelord :Molecular medicine; Gene expression; Antisense oligonucleotides; Positron emission tomography; In vivo hybridisation; In vivo biodistribution; Chromogranin-A; 68Ga; RT-PCR; Scavenger receptors; Molekylärmedicin;

    Sammanfattning : Antisense oligonucleotides (ODN) may interfere in gene expression on the basis of hybridising to its complementary messenger RNA (mRNA) sequence in the cell thereby preventing the synthesis of the peptide. Therefore, these ODNs may be potential drugs to treat human diseases by “knocking down” the expression of responsible genes or correcting the maturation process of mRNA in the field called antisense therapy. LÄS MER

  3. 7. Cyanobacterial Hydrogen Metabolism - Uptake Hydrogenase and Hydrogen Production by Nitrogenase in Filamentous Cyanobacteria

    Författare :Pia Lindberg; Peter Lindblad; Kornél Kovács; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Microbiology; cyanobacteria; biohydrogen; Nostoc; hydrogen production; hydrogen evolution; hydrogen metabolism; nitrogenase; hydrogenase; hydrogen; uptake hydrogenase; hupSL; hydrogen uptake mutant; uptake hydrogenase mutant; photobioreactor; hydrogenase accessory genes; hyp; transcription; RT-PCR; NtcA; mass spectrometry; heterocyst; Mikrobiologi; Microbiology; immunology; infectious diseases; Mikrobiologi; immunologi; infektionssjukdomar;

    Sammanfattning : Molecular hydrogen is a potential energy carrier for the future. Nitrogen-fixing cyanobacteria are a group of photosynthetic microorganisms with the inherent ability to produce molecular hydrogen via the enzyme complex nitrogenase. This hydrogen is not released, however, but is recaptured by the bacteria using an uptake hydrogenase. LÄS MER

  4. 8. Development of Molecular Biology and Bioinformatics Tools : From Hydrogen Evolution to Cell Division in Cyanobacteria

    Författare :Fernando Lopes Pinto; Peter Lindblad; Röbbe Wünschiers; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Cyanobacteria; hydrogen; RNA; bioinformatics; RT-PCR; RACE; transcription; Molecular biology; Molekylärbiologi; Bioinformatics; Bioinformatik; Molecular Biology; Molekylärbiologi;

    Sammanfattning : The use of fossil fuels presents a particularly interesting challenge - our society strongly depends on coal and oil, but we are aware that their use is damaging the environment. Currently, this awareness is gaining momentum, and pressure to evolve towards an energetically cleaner planet is very strong. LÄS MER

  5. 9. Human intraepithelial lymphocytes : a comparative study of phenotype, morphology, and functional properties of intraepithelial lymphocytes in gut and oral mucosa

    Författare :Carina Lundqvist; Marie-Louise Hammarström; Sten Hammarström; Per Brandtzaeg; Umeå universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; human intraepithelial lymphocytes; alimentary tract; intestinal epithelium; gut luminal antigens; gingiva; γδ T cells; immunoelectron microscopy; cytokine mRNA; RT-PCR; extrathymic T cell maturation; RAG-1; periodontitis; mucosal immune system; CD4-CD8- αβ T cells; CD8 γδ T cells;

    Sammanfattning : Human intraepithelial lymphocytes (IEL) constitute a unique cell population situated in the first line of defense of the alimentary tract. Here they are continuously exposed to a massive antigenic load of high complexity. However, different conditions prevail along the alimentary tract. LÄS MER

  7. 10. SUBLINGUAL VACCINATION AGAINST HELICOBACTER PYLORI INFECTION-MUCOSAL IMMUNE RESPONSES AND CORRELATES TO PROTECTIVE IMMUNTY

    Författare :Anna Karin Ostberg; Göteborgs universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Helicobacter pylori; vaccination; sublingual; cytokines; RT-PCR; chemokines; IL-17;

    Sammanfattning : .... LÄS MER