Sökning: "RNase H"

Visar resultat 16 - 20 av 22 avhandlingar innehållade orden RNase H.

  1. 16. Theoretical prediction of ionisation properties of proteins

    Författare :Assen Koumanov; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : This work emphasises on elaboration of improved theoretical and computational methods for determination of protonation/deprotonation equilibria in proteins. It also aims to contribute for a better understanding on atomic level of ionisation properties of proteins and their role in structure-functional relationship. LÄS MER

  3. 17. Studies on phosphate ester cleavage and development of oligonucleotide based artificial nucleases (OBAN’s)

    Författare :Hans Åström; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Five different Oligonucleotide Based Artificial Nuclease (OBAN) systems have been synthesized. OBAN's may be regarded as a development of traditional antisense methodology where inhibition of gene expression can be achieved by hybridizing a synthetic oligonucleotide to natural mRNA and thus inhibiting further translation into protein products. LÄS MER

  4. 18. Mechanistic characterization of post-transcriptional gene silencing

    Författare :Cecilia Dahlgren; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : With the ongoing task of identifying and characterizing genes in the human genome, there is a great demand for tools to study these genes. Today, reverse genetics is the most effective way to explore gene function. This involves suppression of the gene of interest by a set of manipulation techniques. LÄS MER

  5. 19. Retroviral and related nucleic acid processing enzymes expression and detection : Applications on clinical studies of HIV and HERVs

    Författare :Raymond Awad; Amnon Hizi; Uppsala universitet; []
    Nyckelord :Medical sciences; Reverse transcriptase; endogenous retrovirus; deoxycytidine kinase; HIV; HERVS; MEDICIN OCH VÅRD; MEDICINE; MEDICIN; Medical Virology; medicinsk virologi;

    Sammanfattning : Reverse transcriptase (RT), the expression and detection of the enzyme marking the family Retroviridae, has been studied. A new RT activity assay adapted to 96-well microtitre plates is described. It uses a non- radioactive bromodeoxyuridine triphosphate (BrdUTP) substrate in a solid phase format. The assay was highly sensitive. LÄS MER

  7. 20. Nucleic acid based therapeutic approaches

    Författare :Joacim Elmén; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Nucleic acid therapy comprises several approaches based on nucleic acid as the active therapeutic component to treat human disease. Antisense and short interfering RNA (siRNA) are two gene-silencing techniques in this category. Based on nucleic acid sequence recognition both techniques inhibit gene expression. LÄS MER