Sökning: "RNase H"
Visar resultat 11 - 15 av 22 avhandlingar innehållade orden RNase H.
11. Human immunodeficiency virus type 1 proteinase : regulation of function and catalytic activity
Sammanfattning : HIV-l proteinase activity is a prerequisite for viral replication, and one of the main targets for anti-HlV therapy. Although potent proteinase inhibitors are currently being approved for treatment of AIDS patients, these drugs are unlikely to provide treatment which can give patients a life of normal quality and duration. LÄS MER
12. RNA systems for NMR studies in vitro and in vivo
Sammanfattning : NMR spectroscopy is an excellent tool to study the structure-function relationship of RNA. Such measurements are usually performed in vitro, which requires large amounts of isotope- labeled sample in high purity and can give access to individual atoms, structure of the molecule and conformational dynamics. LÄS MER
13. Development of oligonucleotide based artificial ribonucleases 2'-O-MeOBAN´s and PNAzymes
Sammanfattning : The present thesis is based on three parts. The first and second part describe the development of two different classes of metal-ion dependent artificial ribonucleases, 2'-O-methyl-ribonucleic acid based artificial ribonucleases (2'-O-MeOBANs) and peptide nucleic acid based artificial ribonucleases (PNAzymes). LÄS MER
14. HIV-1 reverse transcriptase activity assays based on monoclonal antibodies to native enzyme : Applications on studies of antiviral substances and immunological variation of reverse transcriptase
Sammanfattning : Specific detection of HIV-1 reverse transcriptase (RT) activity in crude materials was enhanced using a capture assay for immunopurification and subsequent activity assay. The purification step was achieved by anti-HIV-1 RT monoclonal antibodies (Mabs) immobilized onto a solid carrier. LÄS MER
15. Novel Pentofuranose Chemistry to Modulate RNA Function
Sammanfattning : Chemical modifications of oligonucleotides provide an important tool to understand how the natural substrate works as well as how to improve their biochemical and biological properties as potential therapeutics and diagnostics. Our carba-LNA (2',4'-carba-bridged Locked Nucleic Acid) modified oligo-DNA or -RNA have been found to be useful to modulate oligo-RNA and -DNA activity. LÄS MER