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Visar resultat 1 - 5 av 22 avhandlingar som matchar ovanstående sökkriterier.

1. 1. EF-Tu and RNase E : Essential and Functionally Connected Proteins

   Författare :Disa L. Hammarlöf; Diarmaid Hughes; Måns Ehrenberg; Gerhart Wagner; Richard H Buckingham; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; bacterial growth; translation; EF-Tu; RNase E; mRNA; RNA degradation; Molekylär cellbiologi; Molecular Cellbiology; Mikrobiologi; Microbiology;

   Sammanfattning : The rate and accuracy of protein production is the main determinant of bacterial growth. Elongation Factor Tu (EF-Tu) provides the ribosome with aminoacylated tRNAs, and is central for its activity. In Salmonella enterica serovar Typhimurium, EF-Tu is encoded by the genes tufA and tufB. LÄS MER

3. 2. A small amoeba at the crossroads of the big RNAi world : MicroRNA biogenesis and Argonaute function in Dictyostelium discoideum

   Författare :Zhen Liao; Fredrik Söderbom; Gerhart E. H. Wagner; Christina Dixelius; Kriton Kalantidis; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; RNAi; microRNA; Argonaute protein; miRNA biogenesis; Dictyostelium discoideum; social amoeba; Dicer; development; growth; Biology with specialization in Molecular Cell Biology; Biologi med inriktning mot molekylär cellbiologi;

   Sammanfattning : Small non-coding RNA (ncRNA) mediated gene silencing, known as RNAi, is a key regulatory mechanism of gene expression in eukaryotes. MicroRNAs (miRNA), one major type of small ncRNAs, are about 21nt long and bound by Argonaute proteins. LÄS MER

4. 3. Targeting RNA by the Antisense Approach and a Close Look at RNA Cleavage Reaction

   Författare :Jharna Barman; Lars Baltzer; Roger Strömberg; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Organic chemistry; mRNA targeting; antisense oligonucleotides; target affinity; RNase H; Michaelis-Menten kinetics; exo-nuclease stability; NMR; pH titration; alkaline hydrolysis; Organisk kemi;

   Sammanfattning : This thesis summarizes the results of studies on two aspects of nucleic acids. Chemically modified antisense oligonucleotides (AONs) have been evaluated with regards to their suitability for mRNA targeting in an antisense approach (Paper I – III). LÄS MER

5. 4. Chemically Modified Oligonucleotides: Synthesis, Physicochemical and Biochemical Properties of their Duplexes with DNA and RNA

   Författare :Pushpangadan Indira Pradeepkumar; Jyoti Chattopadhyaya; Jesper Wengel; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Bioorganic chemistry; Antisense oligonucleotides; Dipyridophenazine; Oxetane; RNase H; Gene down-regulation; DNase 1; Charge transport; Bioorganisk kemi; Bioorganic chemistry; Bioorganisk kemi;

   Sammanfattning : This thesis is based on 9 papers dealing with the synthesis, physicochemical and biochemical properties of two types of chemically modified oligonucleotides which have the potential to down-regulate gene expression: (i) The first set is comprised of antisense oligonucleotides (AONs) conjugated with different chromophores of varying size, charge and π-electron density. Conjugation of the chromophores at the 3'- or 5'-end enhanced the target RNA binding affinity and RNase H recruitment capabilities compared to the native counterpart without changing the global helical conformation of their AON/RNA hybrid duplexes. LÄS MER

7. 5. Conformationally Constrained Nucleosides, Nucleotides and Oligonucleotides : Design, Synthesis and Properties

   Författare :Dmytro Honcharenko; Jyoti Chattopadhyaya; Piet Herdewijn; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Bioorganic chemistry; antisense oligonucleotides; conformationally constrained nucleosides; azetidine; aza-ENA; target affinity; RNase H; exonuclease stability; pyrene-functionalized nucleotides; fluorescence; mismatch discrimination; Bioorganisk kemi; Cell and molecular biology; Cell- och molekylärbiologi;

   Sammanfattning : This thesis is based on six original research publications describing synthesis, structure and physicochemical and biochemical analysis of chemically modified oligonucleotides (ONs) in terms of their potential diagnostic and therapeutic applications. Synthesis of two types of bicyclic conformationally constrained nucleosides, North-East locked 1',2'-azetidine and North locked 2',4'-aza-ENA, is described. LÄS MER