Sökning: "RNAse P"

Visar resultat 1 - 5 av 19 avhandlingar innehållade orden RNAse P.

1. 1. Conformationally Constrained Nucleosides : Design, Synthesis, and Biochemical Evaluation of Modified Antisense Oligonucleotides

   Författare :Oommen P. Varghese; Lars Engman; Jacek Stawinski; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Organic chemistry; chemically modified oligonucleotides; azetidine; aza-ENA-T; cyanomethylene locked; free energy of activation; diaxial interactions; chair conformation; stable duplex; human serum; snake venom phosphodiesterase; antisense agents; RNase H; Organisk kemi; Chemistry; Kemi;

   Sammanfattning : This thesis is concerned with synthesis, structure and biochemical analysis of chemically modified oligonucleotides with potential therapeutic applications. The three types of chemical modifications described here are: (a) A North-East locked 1',2'-azetidine nucleoside (b) A North locked 2',4'-cyanomethylene bridged nucleoside and (c) A 2',4'-aza-ENA-T nucleoside. LÄS MER

3. 2. Investigation of RNase P active site residues and catalytic domain interaction

   Författare :Guanzhong Mao; Leif Kirsebom; Roland Hartmann; Britt-marie Sjöberg; Anders Byström; Anthony Forster; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; RNase P; Ribozyme; PRORP; Induced fit model; RNase P-pre-tRNA interaction; Biology with specialization in Molecular Biology; Biologi med inriktning mot molekylärbiologi;

   Sammanfattning : RNase P is an essential endoribonuclease responsible for the maturation of the tRNA 5’end. The RNase P family encompasses the ribozyme based, RNase P RNP, and proteinaceous RNase P (PRORP). The ribozyme based RNase P is widely distributed in most species while PRORP has so far mainly been found in some eukaryotic cells. LÄS MER

4. 3. Metal ion cooperativity in Escherichia coli RNase P RNA

   Författare :Mathias Brännvall; Britt-Marie Sjöberg; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Cell and molecular biology; RNase P; divalent metal ions; substrate interaction; Cell- och molekylärbiologi; Cell and molecular biology; Cell- och molekylärbiologi; mikrobiologi; Microbiology;

   Sammanfattning : RNase P is an essential ribonuclease responsible for removal of the 5’ leader of tRNA precursors. Bacterial RNase P consists of an RNA subunit and a small basic protein. The catalytic activity is associated with the RNA subunit, i.e. LÄS MER

5. 4. Distal to Proximal—Functional Coupling in RNase P RNA-mediated Catalysis

   Författare :Shiying Wu; Leif A. Kirsebom; Britt-Marie Sjöber; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Ribozyme; RNase P; Induced fit model; tRNA progressing; Substrate interaction;

   Sammanfattning : RNase P is a ubiquitous ribonuclease responsible for removing the 5’ leader of tRNA precursor. Bacterial RNase P contains one RNA (RPR) and one protein (RPP) subunit. However, the number of protein variants depends on the origin. The RNA subunit is the catalytic subunit that in vitro cleaves its substrate with and without the protein subunit. LÄS MER

7. 5. Computational identification of non-coding RNAs

   Författare :Paul Piccinelli; Göteborgs universitet; []
   Nyckelord :RNase P; RNase MRP; IRE; non-coding RNA; secondary structure;

   Sammanfattning : A large amount of genomic information is now becoming available. Suitable bioinformatic tools to organize and analyze this vast amount of information are therefore important. In the case of protein genes, the majority of these may be correctly identified using standard search methods that are based on sequence alignment. LÄS MER