Sökning: "RNA"

Visar resultat 21 - 25 av 1583 avhandlingar innehållade ordet RNA.

  1. 21. Spatially Resolved Gene Expression Analysis

    Författare :Michaela Asp; Joakim Lundeberg; Patrik Ståhl; Thierry Voet; KTH; []
    Nyckelord :RNA; RNA-sequencing; transcriptomics; spatial transcriptomics; single cells; Bioteknologi; Biotechnology;

    Sammanfattning : Spatially resolved transcriptomics has greatly expanded our knowledge of complex multicellular biological systems. To date, several technologies have been developed that combine gene expression data with information about its spatial tissue context. LÄS MER

  3. 22. Lead(II) as a Tool for Probing RNA Structure in vivo

    Författare :Magnus Lindell; Gerhart Wagner; Roland Hartmann; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Molecular biology; lead II cleavage; RNA structure; in vivo probing; Molekylärbiologi; Molecular biology; Molekylärbiologi;

    Sammanfattning : Chemical modification and limited enzymatic hydrolysis are powerful methods to obtain detailed information on the structure and dynamics of RNAs in solution. In the work presented here I have taken advantage of the properties of the divalent metal ion lead(II) to establish it as a new probe for investigating the structure of RNA in vivo. LÄS MER

  4. 23. Ribosome and ribosomal RNA Structure : An experimental and computational analysis of expansion segments in eukaryotic ribosomal RNA

    Författare :Gunnar Alkemar; Odd Nygård; Anders Virtanen; Stockholms universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; eukaryotes; expansion segment; ribosomes; rRNA; secondary structure; structure of rRNA.; Cell biology; Cellbiologi; cellbiologi; Cellbiology;

    Sammanfattning : Ribosomes are large ribonucleoprotein complexes which incorporate amino acids into peptide chains during translational process in all types of living cells. The eukaryotic ribosome is larger compared to its prokaryotic counterpart. LÄS MER

  5. 24. Nucleic Acids as Drug Targets - Influence of Platinum Complexes on Duplex Stability and Binding Kinetics

    Författare :Christopher Polonyi; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Anticancer drugs; platinum complexes; cisplatin; oxaliplatin; nucleic acid; RNA; DNA; duplex melting; thermodynamic stability; kinetics; non-coding RNA; miRNA; siRNA; wobble base pair; loop; BRCA1; mimic; luciferase assay;

    Sammanfattning : Gaining a detailed understanding of how human cells are affected by metal-based drugs is crucial, since these drugs have shown great potential in cancer treatment. Cisplatin is used to treat many different types of cancer, e.g. testicular, ovarian, melanoma, bladder, non-small cell lung cancer, small cell lung cancer, myelomas and lymphomas. LÄS MER

  7. 25. Endogenous Retroviral RNA Expression in Humans

    Författare :Lijuan Hu; Jonas Blomberg; Göran Friman; Francois Mallet; Uppsala universitet; []
    Nyckelord :Microbiology; Human endogenous retrovirus HERV ; HERVE; HERVW; betaretrovirus; gammaretrovirus; RNA expression; real time PCR; endometrium; endometriosis; brain tissue; reproductive tissue; ovarian cancer; ovary; neuroblastoma cell line; Mikrobiologi;

    Sammanfattning : Human endogenous retroviruses (HERVs) constitute about 8% of the human genome. There are around 4000 pol-containing retroviral integrations in the human genome, which makes it impractical to measure each of them separately. LÄS MER