Sökning: "RNA extraction"

Visar resultat 1 - 5 av 26 avhandlingar innehållade orden RNA extraction.

  1. 1. Spatially Resolved Gene Expression Analysis

    Författare :Michaela Asp; Joakim Lundeberg; Patrik Ståhl; Thierry Voet; KTH; []
    Nyckelord :RNA; RNA-sequencing; transcriptomics; spatial transcriptomics; single cells; Bioteknologi; Biotechnology;

    Sammanfattning : Spatially resolved transcriptomics has greatly expanded our knowledge of complex multicellular biological systems. To date, several technologies have been developed that combine gene expression data with information about its spatial tissue context. LÄS MER

  3. 2. Advancing antiviral strategies against emerging RNA viruses by phenotypic drug discovery

    Författare :Marianna Tampere; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Pathogenic RNA viruses can emerge from unexpected sources at unexpected times and cause severe disease in humans, as exemplified by the ongoing coronavirus disease 19 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and Ebola virus (EBOV), Crimean-Congo hemorrhagic fever virus (CCHFV) and Zika virus (ZIKV) outbreaks from the past decade. Despite the increasing impact of emerging viruses to health and economy worldwide, our preparedness to stand against these diseases is hampered by the lack of approved and effective antiviral therapies. LÄS MER

  4. 3. Lung-resident, M2e-specific CD4 T cells critically protect during influenza infection

    Författare :Ajibola Omokanye; Göteborgs universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; influenza; vaccine; universal; heterosubtypic; M2e; CD4; Th17; ThCTL; tissue-resident; cytotoxic; single-cell RNA-seq;

    Sammanfattning : Memory CD4+ T cells are a critical component of the adaptive immune response to fight infection. However, current vaccines against influenza only focus on the other arm of adaptive immunity—triggering B cells to produce antibodies. LÄS MER

  5. 4. Immuno-affinity interactions in sample preparation and analysis

    Författare :Madalina Tudorache; Centrum för analys och syntes; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; immuno supported liquid membrane ISLM extraction; sample preparation; immunoassay; immuno-affinity interaction; antibody; Analytical chemistry; Analytisk kemi;

    Sammanfattning : Immuno-affinity interactions (antibody-antigen) belong, together with enzyme-inhibitor/substrate/coenzyme; oligosaccharide-lectin; DNA/RNA-protein interactions etc, to the class of bio-recognition. Immuno-affinity is widely used for analysis in immunoassays with numerous applications in a variety fields (i.e. LÄS MER

  7. 5. Quantitative analysis of melanoma transcripts : with emphasis on methodological and biological variation

    Författare :Malin Farnebäck; Håkan Hedman; Linköpings universitet; []
    Nyckelord :MEDICINE; MEDICIN;

    Sammanfattning : The introduction of RT-PCR made it possible to detect circulating tumor cells in melanoma patients by analysis of melanoma-associated transcripts, especially tyrosinase. Since the development of the first PCR method for tyrosinase mRNA, several studies have presented varying results. LÄS MER