Sökning: "RNA degradation"

Visar resultat 1 - 5 av 108 avhandlingar innehållade orden RNA degradation.

  1. 1. Conformationally Constrained Oligonucleotides for RNA Targeting

    Författare :Qing Li; Jyoti Chattopadhyaya; Li-He Zhang; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; conformationally constrained nucleoside; antisense oligonucleotide; RNA affinity; nuclease stability; RNase H; RNA degradation; Chemistry with specialization in Bioorganic Chemistry; Kemi med inriktning mot bioorganisk kemi;

    Sammanfattning : A short oligonucleotide sequence as in a single-stranded antisense oligo nucleotides (AON) or in double-stranded small interfering RNAs (siRNA) can modulate the gene expression by targeting against the cellular mRNA, which can be potentially exploited for therapeutic purposes in the treatment of different diseases. In order to improve the efficacy of oligonucleotide-based drugs, the problem of target affinity, nuclease stability and delivery needs to be addressed. LÄS MER

  3. 2. Arthritogenecity of RNA and its degradation products

    Författare :Fariba Zare; Göteborgs universitet; []
    Nyckelord :dsRNA; ssRNA; virus; arthritis; TLR3; IL-1R; IFNalpha beta receptor; plasminogen; tissue factor; uric acid; inflammation; arthritis; neutrophils;

    Sammanfattning : Viral infections often lead to arthralgias and overt arthritic states. Expression of double stranded RNA (dsRNA) is a common feature of all viruses during their replication and it has been suggested that it is able to induce production of pro-inflammatory cytokines, e.g., interferon-alpha (IFN-alpha). LÄS MER

  4. 3. Analysis of RNA and DNA sequencing data : Improved bioinformatics applications

    Författare :Benjamín Sigurgeirsson; Joakim Lundeberg; Simon Anders; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; RNA sequencing; exome sequencing; bioinformatics; gene expression; differential expression; variant calling; Bioteknologi; Biotechnology;

    Sammanfattning : Massively parallel sequencing has rapidly revolutionized DNA and RNA research. Sample preparations are steadfastly advancing, sequencing costs have plummeted and throughput is ever growing. This progress has resulted in exponential growth in data generation with a corresponding demand for bioinformatic solutions. LÄS MER

  5. 4. Nucleic Acids as Drugs and Drug Targets With Focus on Anticancer Active Platinum Complexes and Small Interfering RNAs

    Författare :Hanna Hedman; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Anticancer drugs; platinum complexes; cisplatin; oxaliplatin; nucleic acid; RNA; DNA; siRNA; thermodynamic stability; mass spectrometry; MALDI-MS; chemical probing; luciferase assay;

    Sammanfattning : To ensure that patients receive a safe and effective treatment, it is important to understand how drugs interact with and affect molecules within a cell. Here, evaluation of platinum based drugs and their ability to interfere with nucleic acid functions is presented. LÄS MER

  7. 5. Stability determinants and decay of Bacillus subtilis mRNAs

    Författare :Gustav Hambraeus; Biologiska institutionen; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi; bacteriology; Microbiology; Bacillus subtilis; mRNA stability; degradation;

    Sammanfattning : Degradation of mRNA is a controlled process which is as important for gene regulation as the synthesis of mRNA. This thesis focuses on mechanisms of mRNA stability and degradation in Bacillus subtilis, the model of Gram-positive bacteria. LÄS MER