Sökning: "Quantitative real-time PCR"

Visar resultat 21 - 25 av 102 avhandlingar innehållade orden Quantitative real-time PCR.

1. 21. Plasma Membrane Proteomics and Functional Characterization & Expression Profiling of Plant Aquaporins

   Författare :Erik Alexandersson; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Växtbiokemi; Plant biochemistry; phosphorylation; barley; GFP; GUS; quantitative real-time reverse transcriptase PCR; microarray; aquaporin; MIP; Arabidopsis; plasma membrane; proteomics;

   Sammanfattning : In order to identify integral and peripheral proteins associated with the plasma membrane (PM), highly purified Arabidopsis PMs were analyzed by mass spectrometry. In total, 238 putative PM proteins were identified, of which 114 were predicted to have transmembrane domains or to be PM-anchored. LÄS MER

3. 22. Standardization and application of quantitative PCR methods in patients with hematological malignancies

   Författare :Maria Malec; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :Polymerase chain reaction; RQ-PCR; Minimal Residual Disease; Acute leukemia; Hodgkin s Lymphoma; cytokines;

   Sammanfattning : Using modem combination chemotherapy regimens, it has been possible to increase the rate of complete remission (CR) in acute lymphoblastic (ALL) and acute myeloid leukemia (AML). The major reason for treatment failure in acute leukemia (AL) is the resistance of leukemic blasts to chemotherapeutic agents, which is accompanied by an inability of the immune system to eradicate residual leukemic cells surviving initial treatment. LÄS MER

4. 23. Methodological studies of eicosanoid enzyme gene expression in vascular endothelium exposed to biomechanical stress

   Författare :Roya Doroudi; Göteborgs universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; prostanoids; endothelium; quantitative RT-PCR; perfusion model; shear stress; intraluminal pressure; immediate-early genes;

   Sammanfattning : Prostaglandins are the major eicosanoids synthesized by vascular endothelial cells, and the vasculature is an important target organ for their bioactive metabolites. In vitro studies have shown that biomechanical forces are important regulators of vascular expression of prostanoid enzymes and release of metabolites. LÄS MER

5. 24. Quantification of gene expression in single cells

   Författare :Martin Bengtsson; Islet cell physiology; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Medicin människa och djur ; Medicine human and vertebrates ; Biomedical sciences; Biomedicinska vetenskaper; quantitative gene expression analysis; single cell PCR; islets of Langerhans;

   Sammanfattning : Studies of apparently homogeneous cell populations and single cells often give highly divergent results. Cells exhibit varying responsiveness to stimuli and gene expression and they are in many aspects stochastic and unpredictable. We have developed a method to measure gene expression quantitatively in individual cells with real-time RT-PCR. LÄS MER

7. 25. Dynamics of Human Leukocyte Antigen-D Related expression in bacteremic sepsis

   Författare :Sara Cajander; Jan Källman; Kristoffer Strålin; Bo Söderquist; Per Åkesson; Örebro universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; monocyte HLA-DR; sepsis; immunosuppression; bloodstream infection; HLA-DRA; CIITA; qRT-PCR;

   Sammanfattning : Monocytic human leukocyte antigen-D related (mHLA-DR) expression determined by flow cytometry has been suggested as a biomarker of sepsisinduced immunosuppression.In order to facilitate use of HLA-DR in clinical practice, a quantitative real-time PCR technique measuring HLA-DR at the transcription level was developed and evalutated. LÄS MER