Sökning: "Quantitative PCR"

Visar resultat 16 - 20 av 238 avhandlingar innehållade orden Quantitative PCR.

1. 16. Improved methodologies for molecular detection and quantification of viruses in food

   Författare :Sofia Persson; Patrik Ellström; Magnus Simonsson; Wim van der Poel; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; foodborne virus; digital PCR; real-time PCR; validation; assay design; food safety; Medicinsk vetenskap; Medical Science;

   Sammanfattning : Foodborne viruses such as norovirus, hepatitis A virus and hepatitis E virus cause a high burden of disease worldwide. Reverse transcription (RT) quantitative real-time PCR (qPCR) is the current standard method for monitoring viral contamination in the food chain. LÄS MER

3. 17. PCR detection of Streptococcus pneumoniae and Haemophilus influenzae in pneumonia patients

   Författare :Guma M. K. Abdeldaim; Björn Herrmann; Jonas Blomberg; Kristoffer Strålin; Birgitta Henriques; Uppsala universitet; []
   Nyckelord :Lower respiratory tract infections; Pneumonia; Streptococcus pneumoniae; Haemophilus influenzae; real-time PCR;

   Sammanfattning : PCR is a rapid, reproducible method for nucleic acid detection. However, this technology displays significant deficiencies when applied in clinical microbiology. This work’s aim was to improve current diagnostics and provide sensitive and quantitative real-time PCRs. LÄS MER

4. 18. Real-time PCR for diagnosis of botulism and quantification of neurotoxin gene expression in Clostridium botulinum

   Författare :Ingrid Artin; Avdelningen för medicinsk mikrobiologi; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; detection; qRT-PCR; Clostridium botulinum; molecular methods;

   Sammanfattning : The objectives of the work presented in this thesis have been to develop molecular methods for (i) detection of Clostridium botulinum in food and clinical samples, and (ii) monitoring the expression of the neurotoxin gene, cnt, during different growth phases and under different growth conditions. For the diagnosis of botulism, a real-time PCR-based method including an internal amplification control for C. LÄS MER

5. 19. Detection and Quantification of Variable Viral RNA by Real-Time PCR Assays

   Författare :Shaman Muradrasoli; Jonas Blomberg; Göran Friman; Maria Brytting; Uppsala universitet; []
   Nyckelord :Virology; Diagnosis of Viral RNA; QPCR; RNA virus; broadly targeted PCR; probe design strategy; Infectious diseases; Medicin; Medicine;

   Sammanfattning : As the area of nucleic acid based technologies develops, so will our understanding of how structural variations in DNA and RNA pathogens are associated with disease. The overall goal of this thesis is the development of broadly targeted measurement techniques for variable viral RNA by Real-Time PCR (here referred to as quantitative reverse transcriptase PCR, QRT-PCR). LÄS MER

7. 20. Molecular Diagnosis of Common Viral Infectious Diseases Based on Real-Time PCR

   Författare :Nahla Mohamed; Jonas Blomberg; Jan Fohlman; Elgh Fredrik; Uppsala universitet; []
   Nyckelord :Molecular biology; Microbiology; Virology; rTth DNA polymerase; UNG; QPCR; RNA virus; broadly targeted PCR; Molekylärbiologi;

   Sammanfattning : Molecular biology has become an integral part of the diagnosis of infectious diseases. Recently, quantitative real-time PCR (QPCR) methods (often in the form of so-called TaqMan® systems) have been developed for the diagnosis of a wide range of infectious diseases; these techniques found valuable clinical application in the diagnosis and evaluation of progress and therapeutic success of viral diseases. LÄS MER