Sökning: "Proteome wide protein production"

Hittade 5 avhandlingar innehållade orden Proteome wide protein production.

1. 1. Proteome wide protein production

   Författare :Hanna Tegel; Sophia Hober; Diethard Mattanovich; KTH; []
   Nyckelord :protein production; Escherichia coli; transcription; promoter; translation; rare codon; high-throughput; screening;

   Sammanfattning : Over a decade after the completion of the human genome, researchers around the world are still wondering what information is hidden in the genome. Although the sequences of all human genes are known, it is still almost impossible to determine much more than the primary protein structure from the coding sequence of a gene. LÄS MER

3. 2. Characterization of Antigenic Properties and High Throughput Protein Purification

   Författare :Johanna Steen; Sophia Hober; Mats Ohlin; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; high throughput; protein purification; sample preparation; antigen solubility; immunogenicity; epitope mapping; antigen design; Bioengineering; Bioteknik; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

   Sammanfattning : To understand the cellular processes, knowledge of the localization and function of proteins are essential. There are several high throughput ventures examining the human proteome. However, there are some bottlenecks in these ventures. For example the production and expression of soluble proteins for analysis. LÄS MER

4. 3. Transcriptome and Proteome Analysis using Signature Tags

   Författare :Charlotta Agaton; KTH; []
   Nyckelord :functional genomics; 3´-end signature tags; pyrosequencing; amplification; PrEST; chromosome 21; polyclonal antibodies; dual expression; affinity purification;

   Sammanfattning : With the full sequence of the human genome now available, anexciting era in biomedical research has started. The sequenceprovides information about all our genes and greatly increasesthe scope to compare genetic activities in different cells, toanalyze genetic variation between individuals and betweendifferent species and, most importantly, to investigatesystematically the whole genome in a gene-by-gene manner, andthus increase our understanding of gene function. LÄS MER

5. 4. Systems biology of mitochondrial dysfunction

   Författare :Florian A Schober; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :;

   Sammanfattning : The human body consumes vast amounts of metabolites that are transformed into one another, modified to useful building blocks and broken down to harvest their energy. Mitochondria are at the core of this metabolic turnover and oxidative phosphorylation provides most cellular ATP in almost all human tissues. LÄS MER

7. 5. Living through hard times: Dispose of or sequester? Plant subcellular strategies for stress resilience

   Författare :Pernilla Elander; Sveriges lantbruksuniversitet; Sveriges lantbruksuniversitet; []
   Nyckelord :LANTBRUKSVETENSKAPER; AGRICULTURAL SCIENCES;

   Sammanfattning : Extreme weather events have become the new normal due to climate change and global warming. This damages crop harvests, threatening global food production. One of the many measures to ensure food security is to develop stress-resilient plants, but to do that we need to understand how plants respond to stress factors. LÄS MER