Sökning: "Protein-stabilization"

Visar resultat 1 - 5 av 7 avhandlingar innehållade ordet Protein-stabilization.

  1. 1. Protein stabilization. Some methods and mechanisms

    Författare :Maria Andersson; Bioteknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; sorbitol; protein stability; preferential exclusion; polyol; poly-NIPAAm; polymer; poly ethyleneimine ; polyelectrolyte; oxidation; NMR spectroscopy; lysozyme; lactate dehydrogenase; fluorescence; dynamic light scattering; complex; additive; circular dichroism; stabilization; trypsin; thermo-reversible polymer.; Biotechnology; Bioteknik;

    Sammanfattning : The effect of different additives on protein stability was studied, and the basis for stabilization was investigated at molecular level. The addition of sorbitol enhanced thermal stability of hen egg white lysozyme in aqueous solution. LÄS MER

  3. 2. The Role of Sugars for Protein Stabilization

    Författare :Christoffer Olsson; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; protein; water; QENS; biomolecule; EPSR; amorphous; trehalose; neutron scattering; cryopreservation; DSC; neutron diffraction; dielectric spectroscopy;

    Sammanfattning : The understanding of biomolecular interactions with water and co-solutes can lead to greater knowledge regarding the mechanisms behind biomolecular stabilization. This is highly important for developing technologies aimed to preserve biological materials. LÄS MER

  4. 3. On the Development of Mucin-based Biomaterial Coatings

    Författare :Tomas Sandberg; Karin Caldwell; Jan Carlsson; Thomas Arnebrant; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Mucin; Biomaterial; Surface-exposed protein; XPS; Neutrophil; Cell morphology; SEM; QCM-D; Viscoelasticity; Protein-stabilization; HNL; Coating; MG1; BSM; PGM; SEC-MALS-RI; Mucin quantification; Protein adsorption; Ellipsometry; Biomaterials; Biomaterial;

    Sammanfattning : Owing to their key role in mucosal functioning as surface barriers with biospecific interaction potentials, the mucins are interesting candidates for use as surface modifiers in biomaterials applications. In this work, “mild” fractionation procedures were used to prepare mucins of bovine (BSM), porcine (PGM), and human (MG1) origin. LÄS MER

  5. 4. Unravelling protein stability and aggregation

    Författare :Tanja Weiffert; NanoLund: Centre for Nanoscience; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; protein stability; protein aggregation; amyloid beta peptide; protein stabilization; split GFP; monellin; secretagogin; ; aggregation kinetics; toxicity; aggregation mechanism; coaggregation;

    Sammanfattning : Proteins are complex macromolecules that are fundamental to all living species. The stability of proteins are governed by non-covalent forces within the protein and between the protein and its surrounding environment. LÄS MER

  7. 5. Exercise and regulation of mitochondrial biogenesis factors in human skeletal muscle

    Författare :Jessica Norrbom; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Regular physical activity has many positive implications on health and performance, such as lowered risk for premature death, improved glucose metabolism and cardiovascular function as well as endurance capacity. Even a single bout of exercise is enough stimulus to change expression of many skeletal muscle genes. LÄS MER