Sökning: "Protein expression"

Visar resultat 21 - 25 av 3593 avhandlingar innehållade orden Protein expression.

  1. 21. Vitamin K-dependent protein S - studies of synthesis, structure and function

    Författare :Sofia Somajo; Malmö Klinisk kemi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; C4b-binding protein; coagulation; microparticles; protein C; protein S; structure; tissue factor pathway inhibitor;

    Sammanfattning : This thesis is based on four projects with the overall aim to investigate vitamin K-dependent protein S functions in relation to its structure. The anticoagulant functions of protein S can be divided in three: 1) cofactor for activated protein C (APC), 2) cofactor for tissue factor pathway inhibitor (TFPI) and 3) APC and TFPI-independent anticoagulant functions. LÄS MER

  3. 22. Into the Membrane and Beyond : Improving Membrane Protein Over-Expression in Escherichia coli

    Författare :Mirjam Klepsch; Jan-Willem de Gier; Reinhard Grisshammer; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Escherichia coli; membrane protein over-expression; proteomics; T7 RNA polymerase; peptide transport; strain engineering; Biochemistry; Biokemi; Biophysical chemistry; Biofysikalisk kemi; biokemi; Biochemistry;

    Sammanfattning : Membrane proteins fulfil a wide variety of essential functions in the cell and many are (potential) drug targets. Since their natural abundance is usually very low, most membrane proteins have to be over-expressed for functional and structural studies. LÄS MER

  4. 23. Engineering yeast for improved recombinant protein production

    Författare :Yanyan Wang; Chalmers tekniska högskola; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; point mutation; genome-scale modeling; recombinant protein; omics analysis; CRISPR Cas9; protein secretion; Saccharomyces cerevisiae;

    Sammanfattning : Recombinant proteins are broadly used from basic research to therapeutic development and include industrial enzymes and pharmaceutical proteins. The increasing demand for improved production and enhanced quality of recombinant proteins requires robust biotech-based strategies to overcome the limitations of protein extraction from natural sources. LÄS MER

  5. 24. Engineering microbial cell factories for protein production

    Författare :Kiavash Mirzadeh; Daniel Daley; Grzegorz Kudla; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Protein production; expression vector; recombinant DNA; Translation initiation region; Escherichia coli; mRNA secondary structure; Synthetic evolution; Biochemistry; biokemi;

    Sammanfattning : Proteins are often produced using microbial cell factories for academic or industrial purposes. Protein production is however not an open-and-shut procedure. Production yields often vary in an unpredictable and context dependent manner, limiting the rational design of a straightforward production experiment. LÄS MER

  7. 25. Expression and purification of membrane proteins: Focus on the G-protein coupled receptor MC4r

    Författare :Viveka Dolby; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; enzymology; Proteiner; enzymologi; Proteins; melanocortin 4 receptor; GPCR; purification; Expression; membrane proteins;

    Sammanfattning : Membrane proteins are crucial components of the cell and are involved in many biological processes. Recombinant overexpression systems together with different purification methods are necessary to obtain large amounts of purified receptor for biophysical and functional studies. LÄS MER