Sökning: "PrEST"

Visar resultat 1 - 5 av 8 avhandlingar innehållade ordet PrEST.

  1. 1. Transcriptome and Proteome Analysis using Signature Tags

    Författare :Charlotta Agaton; KTH; []
    Nyckelord :functional genomics; 3´-end signature tags; pyrosequencing; amplification; PrEST; chromosome 21; polyclonal antibodies; dual expression; affinity purification;

    Sammanfattning : With the full sequence of the human genome now available, anexciting era in biomedical research has started. The sequenceprovides information about all our genes and greatly increasesthe scope to compare genetic activities in different cells, toanalyze genetic variation between individuals and betweendifferent species and, most importantly, to investigatesystematically the whole genome in a gene-by-gene manner, andthus increase our understanding of gene function. LÄS MER

  3. 2. Selection of antigens for antibody-based proteomics

    Författare :Lisa Berglund; Mathias Uhlén; Sören Brunak; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; epitope; antigen; antibody; affinity; protein; proteome; proteomics; bioinformatics; prediction; primer design; sequence similarity; Bioengineering; Bioteknik;

    Sammanfattning : The human genome is predicted to contain ~20,500 protein-coding genes. The encoded proteins are the key players in the body, but the functions and localizations of most proteins are still unknown. LÄS MER

  4. 3. Computational analyses of biological sequences -applications to antibody-based proteomics and gene family characterization

    Författare :Mats Lindskog; Mathias Uhlén; Fredrik Sterky; Andersson Björn; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; antigen; antibodies; antibody-based proteomics; biocomputing; bioinformatics; Bioinformatics; Bioinformatik;

    Sammanfattning : Following the completion of the human genome sequence, post-genomic efforts have shifted the focus towards the analysis of the encoded proteome. Several different systematic proteomics approaches have emerged, for instance, antibody-based proteomics initiatives, where antibodies are used to functionally explore the human proteome. LÄS MER

  5. 4. Generation and characterization of antibodies for proteomics research

    Författare :Karin Larsson; Sophia Hober; John McCafferty; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; antibody; antibody validation; immunohistochemistry; immunofluorescence; tissue microarray; Western blot; epitope mapping; antigen design; Bioengineering; Bioteknik;

    Sammanfattning : Specific antibodies are invaluable tools for proteomics research. The availability of thoroughly validated antibodies will help to improve our understanding of protein expression, localization and function; fundamental processes and features of all living organisms. LÄS MER

  7. 5. Transcriptional Analysis of Cell-Sorted B-cell Lymphomas From High-throughput to Focused Identification of Molecular Targets

    Författare :Ulrika Andreasson; Institutionen för immunteknologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; microarray; molecular target; gene expression profiling; B-cell lymphoma; Fluorescence-activated cell sorting;

    Sammanfattning : B cell lymphomas (BCLs) are a group of severe cancers, afflicting both men and women at different ages. Gene expression profiling (GEP), have during the latest decades fundamentally contributed to a better classification and biological understanding of the different BCLs. LÄS MER