Sökning: "Phage display technology"

Visar resultat 11 - 15 av 48 avhandlingar innehållade orden Phage display technology.

  1. 11. Rational and combinatorial genetic engineering approaches for improved recombinant protein production and purification

    Författare :Nina Bandmann; Per-Åke Nygren; Helena Berglund; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Aqueous two-phase system; combinatorial library; expression vector; flow cytometry; fusion tag; partitioning; peptide library; phage display; recombinant proteins; ribosomal binding site; translation initiation; Bioengineering; Bioteknik;

    Sammanfattning : The bacterium Escherichia coli (E. coli) is in many situations an ideal host for production of recombinant proteins, since it generally provides a rapid and economical means to achieve sufficiently high product quantities. LÄS MER

  3. 12. On microchannel acoustophoresis - Experimental considerations and life science applications

    Författare :Per Augustsson; Avdelningen för Biomedicinsk teknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; microtechnology; microfluidics; lab on a chip; separation; acoustophoresis; ultrasound; acoustic resonance; acoustic radiation force; phage display; circulating tumour cells; acoustofluidics;

    Sammanfattning : This thesis presents experimental studies of microchannel acoustophoresis, a technique for manipulation of microscopic objects in suspension by means of acoustic radiation forces induced by ultrasonic standing wave fields. By arranging an acoustic resonance across the width of a microchannel, the path of individual cells or microparticles can be deflected orthogonally to the fluid flow. LÄS MER

  4. 13. Protein Engineering by Directed Evolution and Rational Design

    Författare :Torbjörn Gräslund; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; ion-exchange chromatography; subtilisin; Klenow DNA polymerase; Taq DNA polymerase; directed evolution; rational design; charge engineering; phosphonylating inhibitor; phage display; circular dichroism; protein A; Bioengineering; Bioteknik;

    Sammanfattning : .... LÄS MER

  5. 14. The CD40 Receptor - Target,Tool and Technology

    Författare :Peter Ellmark; Institutionen för immunteknologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Immunology; serology; transplantation; Immunologi; serologi; SPIRE; mammalian; library; cDNA; selection; receptor revision; phage display; scFv; human; CD40; antibodies;

    Sammanfattning : CD40 is a cell surface receptor of pivotal importance that is expressed on several of the cells in the immune system. It is critical for many important events, such as T cell dependent B cell activation, isotype switching, somatic mutation and generation of B cell memory. LÄS MER

  7. 15. Protein–DNA Recognition : In Vitro Evolution and Characterization of DNA-Binding Proteins

    Författare :Mikael Nilsson; Mikael Widersten; Dan Tawfik; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; In vitro evolution; Phage display; mRNA display; Cro; DNA recognition; repressor; Biokemi; Biochemistry; Biokemi;

    Sammanfattning : DNA-recognizing proteins are involved in a multitude of important life-processes. Therefore, it is of great interest to understand the underlying mechanisms that set the rules for sequence specific protein–DNA interactions. Previous attempts aiming to resolve these interactions have been focused on naturally occurring systems. LÄS MER