Sökning: "Periplasmic"

Visar resultat 1 - 5 av 45 avhandlingar innehållade ordet Periplasmic.

1. 1. Growth rate control of periplasmic product retention in Escherichia coli

   Författare :Emma Bäcklund; Gen Larsson; Stefan Schmidt; Amelie Eriksson Karlström; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Escherichia coli; fed-batch; outer membrane proteins; recombinant proteins; specific growth rate; periplasmic retention; phosphotransferase system; high cell density cultivation; acetate formation; Bioengineering; Bioteknik;

   Sammanfattning : The recombinant product is secreted to the periplasm in many processes where E. coli is used as host. One drawback with secretion is the undesired leakage of the periplasmic products to the medium. The aim of this work was to find strategies to influence the periplasmic retention of recombinant products. LÄS MER

3. 2. Impact of glucose uptake rate on recombinant protein production in Escherichia coli

   Författare :Emma Bäcklund; Gen Larsson; Olle Holst; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; AIDA-autotransporter; Escherichia coli; fed-batch; glucose uptake rate; integral membrane proteins; outer membrane proteins; periplasmic retention; phosphotransferase system; recombinant proteins; specific growth rate; surface expression; Biology; Biologi;

   Sammanfattning : Escherichia coli (E. coli) is an attractive host for production of recombinant proteins, since it generally provides a rapid and economical means to achieve high product quantities. LÄS MER

4. 3. Structural Studies of Binding Proteins: Investigations of Flexibility, Specificity and Stability

   Författare :Ulrika Magnusson; Sherry L. Mowbray; Anthony J. Wikinson; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Molecular biology; Periplasmic binding protein; X-ray crystallography; conformational change; acidophile; protein specificity; protein structure; Molekylärbiologi; Molecular biology; Molekylärbiologi;

   Sammanfattning : Binding proteins are present both in gram-negative and gram-positive bacteria. They are the recognition components of the ABC transport systems that transport different nutrients into the cell, and are in some cases also involved in chemotaxis. LÄS MER

5. 4. Protein trafficking in the cell envelope of Escherichia coli : Identification and characterisation of a novel chaperone

   Författare :Hansjörg Götzke; Daniel Daley; Hans-Georg Koch; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; biokemi;

   Sammanfattning : The cell envelope of Gram-negative bacteria, like Escherichia coli, is composed of a cytoplasmic membrane, a periplasmic space containing a peptidoglycan layer and an outer membrane. About 30 % of all proteins are localised in the cell envelope. LÄS MER

7. 5. Production and folding of proteins in the periplasm of Escherichia coli

   Författare :Rageia Elfageih; Jan-Willem de Gier; Hans-Georg Koch; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Escherichia coli; periplasm; recombinant protein production; disulfide bond containing proteins; translation initiation region; protein translocation machinery; co-translational folding; neuraminidases; biokemi; Biochemistry;

   Sammanfattning : The Gram-negative bacterium E. coli is the most widely used host for the production of recombinant proteins. Disulfide bond containing recombinant proteins are usually produced in the periplasm of E. coli since in this compartment of the cell - in contrast to the cytoplasm - disulfide bond formation is promoted. LÄS MER