Sökning: "Padlock probes"

Visar resultat 6 - 10 av 27 avhandlingar innehållade orden Padlock probes.

1. 6. Nucleic acid analysis tools : Novel technologies and biomedical applications

   Författare :Iván Hernández-Neuta; Mats Nilsson; Frank Bier; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Nucleic acid analysis; padlock probes; selector probes; rolling circle amplification; microfluidics; single-molecule detection; digital quantification; fluorescence microscopy; mobile phone microscopy; molecular diagnostics; virology; Biochemistry; biokemi;

   Sammanfattning : Nucleic acids are fundamental molecules of living organisms functioning essentially as the molecular information carriers of life. From how an organism is built to how it responds to external conditions, all of it, can be found in the form of nucleic acid sequences inside every single cell of every life form on earth. LÄS MER

3. 7. Nucleic Acid Based Pathogen Diagnostics

   Författare :Michael S. Akhras; Pål Nyrén; Nader Pourmand; Mats Nilsson; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; pathogen diagnostics; antibiotic resistance; connector inversion probes CIPer ; human papillomavirus HPV ; genotyping; global health diagnostic forum; molecular inversion probe MIP ; neisseria gonorrhoeae; padlock probes; pyrosequencing; Biochemistry; Biokemi;

   Sammanfattning : Pathogenic organisms are transmitted to the host organism through all possible connected pathways, and cause a myriad of diseases states. Commonly occurring curable infectious diseases still impose the greatest health impacts on a worldwide perspective. LÄS MER

4. 8. In situ Sequencing : Methods for spatially-resolved transcriptome analysis

   Författare :Marco Mignardi; Mats Nilsson; Xiaoliang Sunney Xie; Stockholms universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Padlock probes; rolling circle amplification; in situ sequencing; spatially-resolved transcriptomics; molecular diagnostics; Biochemistry; biokemi;

   Sammanfattning : It is well known that cells in tissues display a large heterogeneity in gene expression due to differences in cell lineage origin and variation in the local environment at different sites in the tissue, a heterogeneity that is difficult to study by analyzing bulk RNA extracts from tissue. Recently, genome-wide transcriptome analysis technologies have enabled the analysis of this variation with single-cell resolution. LÄS MER

5. 9. RNA-based spatial characterization of cell and tissue heterogeneity

   Författare :Elin Lundin; Mats Nilsson; Rickard Sandberg; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Padlock probes; in situ sequencing; single cell resolution; single nucleotide variant resolution; spatial transcription profiling; Biochemistry; biokemi;

   Sammanfattning : Technical advances in cell biology have revolutionized the field of cell biology. With new technology it is now possible to address scientific questions in cell biology at the molecular level. LÄS MER

7. 10. iLocks: a novel tool for RNA assays with improved specificity

   Författare :Tomasz Krzywkowski; Mats Nilsson; Gunnar von Heijne; Weng-Onn Lui; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; RNA; miRNA; non-coding RNA; padlock probes; rolling circle amplification; invader; single cell; in situ; adenovirus; virology; diagnostics; Biochemistry; biokemi;

   Sammanfattning : The Central Dogma of molecular biology describes a framework for how genetic information is transferred in cells, placing RNA as a messenger between DNA and translated proteins. During the last years, interest in RNA research has grown tremendously due to the increasing understanding and recognition of the importance of RNA in regulation of gene expression, biochemical catalysis, and genome integrity surveillance. LÄS MER