Sökning: "PCR-RFLP"
Visar resultat 1 - 5 av 9 avhandlingar innehållade ordet PCR-RFLP.
1. Patterns of geographic variation in Silene section Elisanthe (Caryophyllaceae): hybridization and migrational history
Sammanfattning : Large-scale patterns of genetic variation in chloroplast (cp) and nuclear DNA in the widespread European herb species Silene latifolia and S. dioica were investigated using cpPCR-RFLPs, non-coding cpDNA sequences, AFLPs and sequences of the internal transcribed spacers (ITS) of nuclear ribosomal DNA (rDNA). LÄS MER
2. Ectomycorrhizal community structure and function in relation to forest residue harvesting and wood ash applications
Sammanfattning : Ectomycorrhizal fungi form symbiotic associations with tree roots and assist in nutrient-uptake and -cycling in forest ecosystems, thereby constituting a most significant part of the microbial community. The aims of the studies described in this thesis were to evaluate the potential of DNA-based molecular methods in below-ground ectomycorrhizal community studies and to investigate changes in ectomycorrhizal communities on spruce roots in sites with different N deposition, and in sites subjected to harvesting of forest residues or application of wood ash. LÄS MER
3. Geographic differentiation and population history in Silene dioica and S. hifacensis: variation in chloroplast DNA and allozymes
Sammanfattning : In this thesis I used allozymes and PCR-RFLP analysis of chloroplast DNA (cpDNA) to investigate geographic differentiation within the widespread Silene dioica (L.) Clairv. (Caryophyllaceae) and the narrowly-distributed S. hifacensis Rouy ex Willk. LÄS MER
4. Evolutionary history and chloroplast DNA variation in three plant genera: Betula, Corylus and Salix. : The impact of post-glacial colonisation and hybridisation
Sammanfattning : The great difference in the level of chloroplast variation and its geographic structure among the three main species studied here demonstrates that forest species do not form a homogeneous group. Hazel shows a genetic structure similar to many other thermophilous species and this structure, in combination with fossil evidence, indicates that the post-glacial colonisation of most of Europe originated in a refugium in western France while the Balkan and Italy were colonised from a south-eastern refugium. LÄS MER
5. Serodiagnosis and seroprevalence of Helicobacter pylori infection in Vietnam
Sammanfattning : Aims: The aims of the study were to establish and evaluate the best eradication treatment, the optimal diagnostic methods and the impact of Helicobacter pylori infection in Vietnam. Materials: In a treatment trial of patients with peptic ulcer disease (PUD), gastric biopsies and serum samples were obtained from 296 PUD patients (age 18-88 years). LÄS MER