Sökning: "PCR DNA"

Visar resultat 21 - 25 av 420 avhandlingar innehållade orden PCR DNA.

1. 21. Inflammatogenic properties of endogenous DNA

   Författare :Shahin Hajizadeh; Göteborgs universitet; []
   Nyckelord :Inflammation; mtDNA; oxidized DNA; SF; CSF; RA; dementia;

   Sammanfattning : Bacterial DNA and CpG-containing oligodeoxynucleotides (ODNs) induce host inflammatory responses as a result of their content of unmethylated ¡§CpG motifs¡¨. Since the CpG motifs in the mitochondrial (mt) genome are in this respect similar to bacterial DNA, in that they are non-methylated, we investigated whether mtDNA also displays inflammatogenic potential. LÄS MER

3. 22. Genetic Analyses using Rolling Circle or PCR Amplified Padlock Probes

   Författare :Johan Banér; Sanjay Tyagi; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Molecular medicine; Padlock probes; rolling circle amplification; PCR; genotyping; microarray; expression analysis; Molekylärmedicin; Molecular medicine genetics and pathology ; Molekylär medicin genetik och patologi ; Medical Genetics; medicinsk genetik;

   Sammanfattning : Padlock probes are useful in a variety of genetic applications, some of which require that the probes are amplified in order to generate detectable signals. Two general padlock amplification methods, RCA and PCR, are discussed in this thesis. LÄS MER

4. 23. Arrayed identification of DNA signatures

   Författare :Max Käller; Lundeberg Joakim; Mats Nilsson; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; apyrase; allele-specific extension; competitive hybridization; DNA sequencing; genotyping; human papillomavirus HPV ; MC1R; microarray; mutation; p53; protease; Bioengineering; Bioteknik;

   Sammanfattning : In this thesis techniques are presented that aim to determine individual DNA signatures by controlled synthesis of nucleic acid multimers. Allele-specific extension reactions with an improved specificity were applied for several genomic purposes. Since DNA polymerases extend some mismatched 3’-end primers, an improved specificity is a concern. LÄS MER

5. 24. Electric DNA arrays for determination of pathogenic Bacillus cereus

   Författare :Yanling Liu; Sven-Olof Enfors; Helena Anderson; KTH; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Bacillus cereus; haemolysin; non-haemolytic enterotoxin; cytotoxin K-2; cereulide; toxin-coding genes; bacterial colony; electric DNA chip; ultrasonication; DNA fragmentation; Biochemistry; Biokemi;

   Sammanfattning : Silicon-based electric chip arrays were developed for characterization of Bacillus cereus with respect to the capacity to produce toxins involved in food poisoning and foodborne infections. Bacteria of the B. cereus group contain different sets of four toxins encoded by eight genes. LÄS MER

7. 25. Kinetics Quality Assessment for real-time PCR

   Författare :Tzachi Bar; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; kinetics quality assessment; PCR quality assessment; outlier detection; real-time PCR;

   Sammanfattning : For proper DNA quantification by real-time PCR, compared samples should have similar PCR efficiencies. This assumption might not be always correct, as suggested by hundreds of publications dealing with PCR inhibition. LÄS MER