Sökning: "PCR DNA"

Visar resultat 16 - 20 av 420 avhandlingar innehållade orden PCR DNA.

  1. 16. Strategies for de novo DNA sequencing

    Författare :Anna Blomstergren; KTH; []
    Nyckelord :DNA sequencing; DNA purification; automation; solid-phase; streptavidin; biotin; modular probes; Helicobacter pylori; cag PAI;

    Sammanfattning : The development of improved sequencing technologies hasenabled the field of genomics to evolve. Handling andsequencing of large numbers of samples require an increasedlevel of automation in order to obtain high throughput andconsistent quality. LÄS MER

  3. 17. Quantitative detection of bacterial DNA in whole blood in bloodstream infection

    Författare :Ingrid Ziegler; Kristoffer Strålin; Paula Mölling; Magnus Rasmussen; Örebro universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Bloodstream infection; bacteremia; sepsis; DNA load; quantitative PCR; droplet digital PCR; 16S rDNA;

    Sammanfattning : This thesis aims to increase the knowledge on how quantitative PCR can be used in the diagnostics of bloodstream infections, with an emphasis on quantitative elements.In Papers I and II, we evaluated quantitative data from two commercial PCR tests for pathogen detection directly in blood, Magicplex Sepsis (I) and SeptiFast (II), from patients with suspected sepsis. LÄS MER

  4. 18. Selector Technology : For Multiplex DNA Analysis

    Författare :Fredrik Dahl; Mats Nilsson; Ulf Landegren; Ivo Glynne Gut; Uppsala universitet; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Genetic engineering; DNA technology; Genteknik; Genetic engineering including functional genomics; Genteknik inkl. funktionsgenomik;

    Sammanfattning : A majority of methods for identifying sequences in the human genome involve target sequence amplification through PCR. This work presents novel methods for amplifying circularized DNA and presents solutions for some major limitations of PCR. LÄS MER

  5. 19. Methods to Prepare DNA for Efficient Massive Sequencing

    Författare :Sverker Lundin; Joakim Lundeberg; Harold Swerdlow; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; DNA; Massive sequencing; Next Generation Sequencing; Library Preparation; Barcoding; Multiplexing;

    Sammanfattning : Massive sequencing has transformed the field of genome biology due to the continuous introduction and evolution of new methods. In recent years, the technologies available to read through genomes have undergone an unprecedented rate of development in terms of cost-reduction. LÄS MER

  7. 20. Molecular analysis of the oral microbiota of dental diseases

    Författare :Eleni Kanasi; Sirkka Asikainen; Anne CR Tanner; Ingegerd Johansson; Gunnar Dahlén; Umeå universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; early childhood caries; early periodontitis; 16S rDNA cloning and sequencing; whole genomic DNA probes; oligonucleotide DNA probes; PCR; diversity; molecular; aggregatibacter actinomycetemcomitans; serotypes; Oral microbiology; Oral mikrobiologi;

    Sammanfattning : Traditionally, bacterial culture has been used for bacterial detection, allowing study of living microorganisms. Molecular methods are rapid and allow simultaneous identification of numerous species and uncultivated phylotypes. LÄS MER