Sökning: "Overexpression"

Visar resultat 1 - 5 av 546 avhandlingar innehållade ordet Overexpression.

  1. 1. GFP as a tool to monitor membrane protein topology and overexpression in Escherichia coli

    Författare :David Drew; Jan-Willem de Gier; Gunnar von Heijne; Bert Poolman; Stockholms universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; GFP; membrane protein; topology; overexpression; high-throughput; Biochemistry; Biokemi;

    Sammanfattning : Membrane proteins are essential for life, and roughly one-quarter of all open reading frames in sequenced genomes code for membrane proteins. Unfortunately, our understanding of membrane proteins lags behind that of soluble proteins, and is best reflected by the fact that only 0. LÄS MER

  2. 2. Intracellular vesicles induced by monotopic membrane protein in Escherichia coli

    Författare :Hanna M. Eriksson; Åke Wieslander; Meta Kuehn; Stockholms universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Membrane protein; intracellular vesicles; Escherichia coli; glycosyltransferase; overexpression; optimization; detergent; screening; lipid composition; Biochemistry; Biokemi; biokemi; Biochemistry;

    Sammanfattning : The monotopic membrane protein alMGS, a glycosyltransferase catalyzing glucolipid synthesis in Acholeplasma laidlawii, was overexpressed in Escherichia coli. Optimization of basic growth parameters was performed, and a novel method for detergent and buffer screening using a small size-exclusion chromatography was developed. LÄS MER

  3. 3. The Aquaporins - Expression and Regulation of Water Channel Proteins

    Författare :Maria Karlsson; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Plant biochemistry; immunocytochemistry; overexpression; phosphorylation; aquaporin; water channel; Växtbiokemi; Chemistry; Kemi;

    Sammanfattning : Aquaporins are integral membrane proteins that function as water channels highly specific for water or as aquaglyceroporins permeable to water as well as small solutes such as glycerol and urea. The model plant Arabidopsis thaliana has 35 genes encoding aquaporin proteins. LÄS MER

  4. 4. Human neural stem cells: region-specific properties and prospects for cell therapy

    Författare :Therese Kallur; Lund Neurologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Pax6; fetal cortex and striatum; human; neural stem cells; transplantation; neurospheres; stroke; overexpression; region-specific properties;

    Sammanfattning : Cell replacement by neural transplantation can, in animal models of neurodegenerative diseases, reconstruct damaged brain circuitry. In the clinical situation, the graft material used for cell therapy must, most likely, be of human origin. LÄS MER

  5. 5. Functional studies of a membrane-anchored cellulase from poplar

    Författare :Ulla Jonsson Rudsander; Tuula Teeri; Eva Nordberg-Karlsson; KTH; []
    Nyckelord :ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; Populus; cellulose biosynthesis; endo-1; 4-b-glucanase; cellulase; Pichia pastoris; Arabidopsis thaliana; NMR spectroscopy; cleavage pattern; stereochemistry; transglycosylation; complementation; hybrid proteins; overexpression; substrate analysis; overglycosylation; Bioengineering; Bioteknik;

    Sammanfattning : Cellulose in particular and wood in general are valuable biomaterials for humanity, and cellulose is now also in the spotlight as a starting material for the production of biofuel. Understanding the processes of wood formation and cellulose biosynthesis could therefore be rewarding, and genomics and proteomics approaches have been initiated to learn more about wood biology. LÄS MER