Sökning: "Overexpression"
Visar resultat 1 - 5 av 602 avhandlingar innehållade ordet Overexpression.
1. GFP as a tool to monitor membrane protein topology and overexpression in Escherichia coli
Sammanfattning : Membrane proteins are essential for life, and roughly one-quarter of all open reading frames in sequenced genomes code for membrane proteins. Unfortunately, our understanding of membrane proteins lags behind that of soluble proteins, and is best reflected by the fact that only 0. LÄS MER
2. Intracellular vesicles induced by monotopic membrane protein in Escherichia coli
Sammanfattning : The monotopic membrane protein alMGS, a glycosyltransferase catalyzing glucolipid synthesis in Acholeplasma laidlawii, was overexpressed in Escherichia coli. Optimization of basic growth parameters was performed, and a novel method for detergent and buffer screening using a small size-exclusion chromatography was developed. LÄS MER
3. The Aquaporins - Expression and Regulation of Water Channel Proteins
Sammanfattning : Aquaporins are integral membrane proteins that function as water channels highly specific for water or as aquaglyceroporins permeable to water as well as small solutes such as glycerol and urea. The model plant Arabidopsis thaliana has 35 genes encoding aquaporin proteins. LÄS MER
4. Human neural stem cells: region-specific properties and prospects for cell therapy
Sammanfattning : Cell replacement by neural transplantation can, in animal models of neurodegenerative diseases, reconstruct damaged brain circuitry. In the clinical situation, the graft material used for cell therapy must, most likely, be of human origin. LÄS MER
5. Functional studies of a membrane-anchored cellulase from poplar
Sammanfattning : Cellulose in particular and wood in general are valuable biomaterials for humanity, and cellulose is now also in the spotlight as a starting material for the production of biofuel. Understanding the processes of wood formation and cellulose biosynthesis could therefore be rewarding, and genomics and proteomics approaches have been initiated to learn more about wood biology. LÄS MER