Sökning: "Over-expression"

Visar resultat 1 - 5 av 124 avhandlingar innehållade ordet Over-expression.

  1. 1. Into the Membrane and Beyond : Improving Membrane Protein Over-Expression in Escherichia coli

    Detta är en avhandling från Stockholm : Department of Biochemistry and Biophysics, Stockholm University

    Författare :Mirjam Klepsch; Stockholms universitet.; [2011]
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Escherichia coli; membrane protein over-expression; proteomics; T7 RNA polymerase; peptide transport; strain engineering; NATURAL SCIENCES Chemistry Biochemistry; NATURVETENSKAP Kemi Biokemi; NATURAL SCIENCES Chemistry Physical chemistry Biophysical chemistry; NATURVETENSKAP Kemi Fysikalisk kemi Biofysikalisk kemi; biokemi; Biochemistry;

    Sammanfattning : Membrane proteins fulfil a wide variety of essential functions in the cell and many are (potential) drug targets. Since their natural abundance is usually very low, most membrane proteins have to be over-expressed for functional and structural studies. LÄS MER

  2. 2. Extracellular serine proteases as virulence factors in nematophagous fungi: Molecular characterization and functional analysis of the PII protease in Arthrobotrys oligospora

    Detta är en avhandling från Microbial Ecology, Lund University

    Författare :Johan Åhman; Lunds universitet.; Lund University.; [2000]
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biology; Aspergillus niger.; Heterologous expression; Over-expression; Gene-disruption; Transformation system; Serine protease; Biological control; Arthrobotrys oligospora; Fungi; Nematophagous; Biologi;

    Sammanfattning : Nematophagous fungi are parasites of nematodes, thus they have been considered and tested for biological control of parasitic nematodes, but so far with limited success. Due to the fact that the nematode cuticle is composed of proteins it is likely that fungal extracellular proteases are involved in the infection of nematodes. LÄS MER

  3. 3. Engineering membrane proteins for production and topology

    Detta är en avhandling från Stockholm : Department of Biochemistry and Biophysics, Stockholm University

    Författare :Stephen Toddo; Stockholms universitet.; [2015]
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Membrane protein; Over-expression; Protein production; Codon optimization; Escherichia coli; AraH; NarK; mRNA secondary structure; coding sequence; ribosome binding site; RBS; topology; split-GFP; biokemi; Biochemistry;

    Sammanfattning : The genomes of diverse organisms are predicted to contain 20 – 30% membrane protein encoding genes and more than half of all therapeutics target membrane proteins. However, only 2% of crystal structures deposited in the protein data bank represent integral membrane proteins. LÄS MER

  4. 4. Role of NFAT (nuclear factor of activated T cells) in vascular smooth muscle

    Detta är en avhandling från Department of Clinical Sciences, Lund University

    Författare :Lisa Berglund; Lunds universitet.; Lund University.; [2009]
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; vascular smooth muscle; proliferation; A-285222; osteopontin; AIF-1; diabetes; vascular disease; calcium; glucose; NFAT;

    Sammanfattning : The calcium-dependent transcription factor NFAT was first described as a key player in immune cell regulation. Since then, NFAT signaling has been shown to be crucial for normal vascular development during embryogenesis. LÄS MER

  5. 5. Metabolic Engineering of the Pentose Phosphate Pathway of Xylose Fermenting Saccharomyces cerevisiae

    Detta är en avhandling från Applied Microbiology (LTH)

    Författare :Björn Johansson; Lunds universitet.; Lund University.; [2001]
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Kemiteknik och kemisk teknologi; Chemical technology and engineering; xylulokinase; pentose phosphate pathway; genetic engineering; Saccharomyces cerevisiae; xylose; Microbiology; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi;

    Sammanfattning : The aim of the work presented in this thesis is the improvement of xylose fermentation performance of recombinant Saccharomyces cerevisiae strains expressing XYL1 and XYL2 from Pichia stipitis, encoding xylose reductase (XR) and xylitol dehydrogenase (XDH). A recombinant strain of S. LÄS MER