Sökning: "Nucleic acid analysis"

Visar resultat 1 - 5 av 59 avhandlingar innehållade orden Nucleic acid analysis.

  1. 1. Nucleic acid analysis tools : Novel technologies and biomedical applications

    Författare :Iván Hernández-Neuta; Mats Nilsson; Frank Bier; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Nucleic acid analysis; padlock probes; selector probes; rolling circle amplification; microfluidics; single-molecule detection; digital quantification; fluorescence microscopy; mobile phone microscopy; molecular diagnostics; virology; Biochemistry; biokemi;

    Sammanfattning : Nucleic acids are fundamental molecules of living organisms functioning essentially as the molecular information carriers of life. From how an organism is built to how it responds to external conditions, all of it, can be found in the form of nucleic acid sequences inside every single cell of every life form on earth. LÄS MER

  3. 2. Molecular Tools for Nucleic Acid Analysis

    Författare :Deirdre O'Meara; KTH; []
    Nyckelord :nucleic acid capture; modular probes; biosensor; bio-magnetic separation; hepatitis C; sequencing; pyrosequencing; mutation detection; HIV-1; drug resistance; SNP; allele-specific extension; apyrase; genotyping.;

    Sammanfattning : Nucleic acid technology has assumed an essential role invarious areas ofin vitrodiagnostics ranging from infectious diseasediagnosis to human genetics. An important requirement of suchmolecular methods is that they achieve high sensitivity andspecificity with a fast turnaround time in a cost-effectivemanner. LÄS MER

  4. 3. Droplet microfluidics for single cell and nucleic acid analysis

    Författare :Prem Kumar Periyannan Rajeswari; Helene Andersson Svahn; Håkan Jönsson; Martin Dufva; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Acoustophoresis; Biomarker detection; Cell behavior analysis; Cell factories; Droplet microfluidics; Droplet PCR; High throughput biology; Label-free enrichment; Single cell analysis; Bioteknologi; Biotechnology;

    Sammanfattning : Droplet microfluidics is an emerging technology for analysis of single cells and biomolecules at high throughput. The controlled encapsulation of particles along with the surrounding microenvironment in discrete droplets, which acts as miniaturized reaction vessels, allows millions of particles to be screened in parallel. LÄS MER

  5. 4. Thermodynamics of Peptide Nucleic Acid Interactions with DNA

    Författare :Tommi Ratilainen; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; thermodynamics; enthalpy-entropy compensation; ionic strength; nucleic acids; fluorescence energy transfer; PNA; calorimetry; sequence-specific binding;

    Sammanfattning : Peptide Nucleic Acid (PNA) is a DNA mimic with the natural nucleobases attached to a charge-neutral pseudopeptide backbone. It has remarkably strong and sequence-specific binding affinity to natural nucleic acids (DNA and RNA), mainly attributed to the lack of charge in the PNA backbone. LÄS MER

  7. 5. Application of Padlock Probe Based Nucleic Acid Analysis In Situ

    Författare :Sara Henriksson; Mats Nilsson; Ulf Landegren; Harvest Gu; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Padlock probe; rolling circle amplification; single cell gel electrophoresis assay; comet assay; Crimean Congo hemorrhagic fever virus; Molecular medicine; Molekylär medicin; Molekylär medicin; Molecular Medicine;

    Sammanfattning : The great variation displayed by nucleic acid molecules in human cells, and the continuous discovery of their impact on life, consequently require continuous refinements of molecular analysis techniques. Padlock probes and rolling circle amplification offer single nucleotide discrimination in situ, a high signal-to-noise ratio and localized detection within cells and tissues. LÄS MER