Sökning: "Negative cell isolation"

Visar resultat 1 - 5 av 27 avhandlingar innehållade orden Negative cell isolation.

  1. 1. Cell motility : the dynamics of the cortical weave of microfilaments and its relation to microtubules and coated vesicles in fibroblasts and glial cells

    Författare :Anna-Stina Höglund; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES;

    Sammanfattning : Actin and myosin, the major components of the microfilament system are found in most - if not all - eukaryotic cells. In muscle they form the basis of the contractile apparatus which generates the rapid, strong and unidirectional movements that characterizes muscle activity. LÄS MER

  3. 2. Magnetic bead-based isolation of biological therapeutic modalities

    Författare :Nils A. Brechmann; Véronique Chotteau; Nigel Titchener-Hooker; KTH; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Magnetic bead separation; Magnetic beads; Protein A; Protein G; Negative cell isolation; Monoclonal antibody; induced pluripotent stem cells; human mesenchymal stem cells; Allogenic cell therapy; Biomanufacturing; cell clarification; Biotechnology; Bioteknologi;

    Sammanfattning : Biopharmaceutical modalities, such as monoclonal antibodies or the less established cell therapies, are nowadays very important for the treatment of severe or incurable diseases. The manufacturing of such modalities is complex and costly, including the downstream processing, which is highly essential to ensure the safety and quality of the product. LÄS MER

  4. 3. Characterization of artificial and biological lipid vesicles using TIRF and SPR

    Författare :Thomas Olsson; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; membrane permeability; chromaffin vesicle; fluorescence; total internal reflection TIRF microscopy; surface plasmon resonance SPR ; vesicle; osmotic shock; cell membrane; liposome; nanoparticle tracking analysis NTA ; large dense core vesicles LDCVs ;

    Sammanfattning : Synthetic lipid vesicles serve as important mimics of cells and the natural membranes that they are enclosed by. As such they are frequently used as simplified models of the highly complex cell membrane to aid in-depth physicochemical and biological characterization of this essential biological structure. LÄS MER

  5. 4. Investigations of Hematopoietic Stem Cells and Their Age-Associated Alterations

    Författare :Alexandra Rundberg Nilsson; Avdelningen för molekylär hematologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Hematopoietic stem cells; Aging; Serial transplantation; Self-renewal; Flow cytometry; Gene expression;

    Sammanfattning : The hematopoietic stem cell (HSC) has and continues to be extensively investigated, and represents by far the most studied somatic stem cell. Development of various experimental technologies, including fluorescence activated cell sorting (FACS), have been crucial for research advancements within the hematopoietic field. LÄS MER

  7. 5. Extracellular vesicles in Shiga toxin-mediated disease

    Författare :Annie Willysson; Lund Pediatrik; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Extracellular vesicles; Shiga toxin; EHEC; hemolytic uremic syndrome;

    Sammanfattning : Extracellular vesicles are released during infectious, inflammatory and prothrombotic conditions. These are membrane-derived vesicles shed by cells during activation, senescence and/or apoptosis. Extracellular vesicles contain and transfer components of the parent cell from which they are shed. LÄS MER