Sökning: "NK-cell culture"

Visar resultat 1 - 5 av 19 avhandlingar innehållade orden NK-cell culture.

  1. 1. Migration of Natural Killer Cells. Matrix interaction, locomotion and regulation of matrix metalloproteinases (MMPs) by IL-2 and chemokines

    Författare :Karin Edsparr; Göteborgs universitet; []
    Nyckelord :NK cell; MMP; Matrigel; migration; IL-2;

    Sammanfattning : Activated natural killer (NK) cells are effective anti-tumour cells. In order to reach target cells in a tumour mass they need to migrate in the extravascular space. LÄS MER

  3. 2. Human cytomegalovirus : development of resistance to antiviral drugs and mechanisms of NK-cell evasion

    Författare :Mehrdad Mousavi-Jazi; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Human cytomegalovirus (HCMV), a member of the herpesviridae, is ubiquitous and causes persistent infections mostly without any clinical symptoms. However, HCMV infection is a major cause of morbidity and mortality among immunocompromised patients, especially transplant recipients and patients with AIDS. LÄS MER

  4. 3. Leukaemic relapse after allogeneic haematopoietic stem cell transplantation and the use of the graft-versus-leukaemia effect

    Författare :Stefan Carlens; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :haematopoietic stem cell transplantation; GVHD; GVL; DLI; T-cell culture; retroviral transduction; NK-cell culture;

    Sammanfattning : Survival after allogeneic haematopoietic stem cell transplantation (HSCT) has improved over the last decades due to improvements in immunosuppressive regimens and supportive care. Today the major risk of treatment failure is recurrent leukaemia, a risk which has stayed relatively constant over the years. LÄS MER

  5. 4. Flow cytometric methods for assessment of cell-mediated immune responses

    Författare :Karina Godoy Ramirez; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Flow cytometry; intracellular cytokine staining ICS ; NK cell; CTL; cytotoxicity; HIV-1;

    Sammanfattning : The aim of this thesis was to develop and improve flow cytometry-based methods for assessment of cellmediated immune responses (CMI) induced by natural infection or vaccination. Traditionally, the measurement of anti genspecific cellular immune responses has been based on the use of complex, labourintensive, and less informative bulkresults assays (51Cr-release, 3 3H-thymidin uptake and Limiting dilution assay, LDA). LÄS MER

  7. 5. Ultrasound-assisted Interactions of Natural Killer Cells with Cancer Cells and Solid Tumors

    Författare :Athanasia Christakou; Martin Wiklund; Sabeth Verpoorte; KTH; []
    Nyckelord :Natural killer cell; cytotoxicity; heterogeneity; multiwell microchip; biocompatibility; ultrasonic cell manipulation; 3D cell culture; solid tumor; spheroid; high-resolution imaging; Biologisk fysik; Biological Physics;

    Sammanfattning : In this Thesis, we have developed a microtechnology-based method for culturing and visualizing high numbers of individual cells and cell-cell interactions over extended periods of time. The foundation of the device is a silicon-glass multiwell microplate (also referred as microchip) directly compatible with fluorescence microscopy. LÄS MER