Sökning: "NATURVETENSKAP Kemi Analytisk kemi Elektrokemi"

Visar resultat 1 - 5 av 6 avhandlingar innehållade orden NATURVETENSKAP Kemi Analytisk kemi Elektrokemi.

  1. 1. Amperometric Biosensors for Detection of Analytes in Cellular Models

    Författare :Jaime Castillo Leon; Centrum för analys och syntes; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Analytical chemistry; Simultaneous detection; Bienzyme electrode; Amperometric biosensors; Cellular models; Analytisk kemi; Electrochemistry; Elektrokemi;

    Sammanfattning : Various biological problems such as disease mechanisms, drug development and toxicological studies motivated the introduction of cellular models that today also represent an alternative for reducing the use of animals in controversial medical and pharmaceutical experiments. Cells are complex matrices where several processes occur simultaneously. LÄS MER

  3. 2. Functional aspects of cellobiose dehydrogenase Applications for biosensor development

    Författare :Leonard Stoica; Centrum för analys och syntes; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Elektrokemi; Electrochemistry; Analytisk kemi; Analytical chemistry; bioelectrochemistry; direct electron transfer; lactose biosensor;

    Sammanfattning : Electrochemcial investigations were conducted for elucidating and understanding the relation between the various electron transfer processes occurring in cellobiose dehydrogenase (CDH) bound to an electrode surface. Substrate inhibition caused by cellobiose was proven to act in competition with the electron transfer to a two-electron acceptor and under a partial inhibition mechanism for the internal electron transfer process. LÄS MER

  4. 3. Amperometric biosensors of food analysis relevance: Emphasis on the neurotoxin beta-ODAP in grass pea

    Författare :Yirgalem Yigzaw; Centrum för analys och syntes; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Elektrokemi; Electrochemistry; Analytisk kemi; Analytical chemistry; neurotoxin; Amperometric biosensors; Lathyrus sativus;

    Sammanfattning : Enzyme-based amperometric biosensors have been developed and characterized in flow systems for a variety of analytes such as [beta]-N-oxalyl-[alfa],[beta]-diamionpropionic acid ([beta]-ODAP), L-glutamate, glucose, lactate, and sucrose, that have food analysis relevance. The principle of enzyme "wiring" using polymeric mediators poly(1-vinylimidazole) {(Os-4, 4' dimethylbipyridyl)2Cl3+/2+} and poly(4-vinlypyridine {Os(N,N'-dimethyl-2,2'-biimidazole)3}3+/2+ has been employed in most of the sensors developed, which resulted in the fabrication of sensitive, stable and reproducible biosensors with carbonaceous electrodes (spectrographic graphite and glassy carbon). LÄS MER

  5. 4. Electrochemistry of Heme Containing Enzymes - Fundamentals and Applications

    Författare :Annika Lindgren; Centrum för analys och syntes; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; phenol monitoring; spectroelectrochemistry; self-assembled monolayer; gold electrode; graphite electrode; direct electron transfer; cellobiose dehydrogenase; biosensor; peroxidase; kinetics; Electrochemistry; Elektrokemi;

    Sammanfattning : The thesis is a bioelectrochemical study of heme enzymes, mainly peroxidases and cellobiose dehydrogenases (CDH). It covers both fundamental and applied aspects, viz. studies of the direct electron transfer and kinetics of enzymes on electrodes, and the development of amperometric enzyme-based biosensors. LÄS MER

  7. 5. Spectroelectrochemistry of Redox Enzymes

    Författare :Andreas Christenson; Centrum för analys och syntes; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Elektrokemi; Electrochemistry; complex II; bilirubin oxidase; laccase; enzyme mechanism; redox potential; electron transfer; spectroelectrochemistry; redox enzymes; Spectroelectrochemical cell;

    Sammanfattning : A low volume and long optical path spectroelectrochemical cell was constructed and tested in a number of applications such as detection of direct electron transfer (DET) between redox enzymes and gold electrodes, determination of the formal potentials of redox centres in proteins and enzymes, as well as mechanistic studies of heterogeneous and intermolecular electron transfer of copper oxidases and succinate:quinine oxidoreductase (Complex II). The existence of DET between enzymes and electrodes was demonstrated for theophylline oxidase, cytochrome P450 cam, laccase, bilirubin oxidase, sulphite oxidase and Complex II. LÄS MER