Sökning: "NADH"

Visar resultat 6 - 10 av 98 avhandlingar innehållade ordet NADH.

  1. 6. Gene fusion in protein engineering : design of novel peptides and bifunctional enzymes

    Författare :Sarah Fredriksson; Tillämpad biokemi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Cadmium; Bifunctional enzyme; Catalytic triad; Channeling; Coenzyme recycling; Galactose dehydrogenase; ß-Galactosidase; Gene fusion; Hexapeptide; Lactate dehydrogenase; Linker region; Multifunctional enzymes; Oligomeric protein; NADH; Phage; OmpA; Biochemistry; Biokemi; metabolism; Biotechnology; Bioteknik;

    Sammanfattning : The gene fusion technique is widely used in protein engineering and in this thesis it constitutes the basis of both the construction of artificial bifunctional enzymes and the display of two novel peptides. Artificial bifunctional enzymes have been used as model systems in the studies of native multifunctional enzymes and proximity effects within these complexes. LÄS MER

  3. 7. Glycolytic oscillations in individual yeast cells

    Författare :Anna-Karin Gustavsson; Göteborgs universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Optical manipulation; Microfluidics; Fluorescence microscopy; Single cell analysis; Saccharomyces cerevisiae; Glycolysis; Oscillations; NADH; Heterogeneity; Synchronization; Robustness; Universality;

    Sammanfattning : Oscillations in the concentration of yeast glycolytic intermediates have been intensively studied since the 1950s, but these studies have so far been limited to observations of average oscillatory behavior in synchronized cultures. Hence, it has remained unknown whether the onset of oscillations is a collective property of the population which requires a high cell density, or if individual cells can oscillate also in isolation. LÄS MER

  4. 8. Single Cell Studies of Glycolytic Oscillations Using Microfluidics and Optical Tweezers

    Författare :Anna-Karin Gustavsson; Göteborgs universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Optical manipulation; microfluidics; fluorescence microscopy; single cell imaging; Saccharomyces cerevisiae; glycolysis; oscillations; NADH;

    Sammanfattning : The complex set of reactions in metabolism has been extensively studied in populations with millions of cells, but much information can still be gained by studying the heterogeneous metabolic behaviour in individual cells. The first part of energy metabolism is called glycolysis and is very similar in mammalian cells, such as human cells, and in yeast cells. LÄS MER

  5. 9. The role of the GPD2 encoded glycerol 3-phosphate dehydrogenase in redox metabolism of SACCHAROMYCES CEREVISIAE

    Författare :Åsa Valadi; Göteborgs universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; GPDI1; GPDI2; NADH; redox; glycerol; mitochondria; metabolism; S. cerevisiae;

    Sammanfattning : .... LÄS MER

  7. 10. Synthesis and Studies of NADH Models: Stereoselective Reductions and Host-Guest Properties

    Författare :Ulrik Gran; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; synthesis; NADH model; host-guest complex; stereoselective reductions; macrocyclic; reducing agent;

    Sammanfattning : The aim of the project has been to synthesise macrocyclic NADH models and to study them as reducing agents and hosts. Three types of macrocyclic NADH models have been synthesised. They consist of the same subunits; a chiral unit, a nicotinamide unit and an aromatic spacer. LÄS MER