Sökning: "NAD NADH recycling"

Hittade 2 avhandlingar innehållade orden NAD NADH recycling.

  1. 1. Gene fusion in protein engineering : design of novel peptides and bifunctional enzymes

    Författare :Sarah Fredriksson; Tillämpad biokemi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Cadmium; Bifunctional enzyme; Catalytic triad; Channeling; Coenzyme recycling; Galactose dehydrogenase; ß-Galactosidase; Gene fusion; Hexapeptide; Lactate dehydrogenase; Linker region; Multifunctional enzymes; Oligomeric protein; NADH; Phage; OmpA; Biochemistry; Biokemi; metabolism; Biotechnology; Bioteknik;

    Sammanfattning : The gene fusion technique is widely used in protein engineering and in this thesis it constitutes the basis of both the construction of artificial bifunctional enzymes and the display of two novel peptides. Artificial bifunctional enzymes have been used as model systems in the studies of native multifunctional enzymes and proximity effects within these complexes. LÄS MER

  3. 2. Bioelectrochemical Applications of Reactions Catalyzed by Immobilized Enzymes

    Författare :Xiao-Jing Tang; Centrum för analys och syntes; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Acetate Kinase.; NAD NADH recycling; Enzyme Electrodes; Enzyme reactors; Enzyme Immobilization; Electrochemical detection; Flow Injection Analysis; Analytical chemistry; Analytisk kemi;

    Sammanfattning : The sensitivity of biosensors can be increased substantially by incorporation in a substrate recycling scheme. Based on the amplification of NAD+/NADH by the recycling reaction catalyzed by glycerol dehydrogenase (GDH) and diaphorase, NAD+/NADH can be determined amperometrically with around 1000 times higher sensitivity than for the substrate sensing mode. LÄS MER