Sökning: "Multiplex PCR"

Visar resultat 1 - 5 av 69 avhandlingar innehållade orden Multiplex PCR.

  1. 1. Diagnostic PCR for the Detection of Yersinia enterocolitica and Salmonella in the Food-Chain: Reliability of PCR Performance

    Författare :Rickard Knutsson; Teknisk mikrobiologi; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Livsmedelsteknik; Food and drink technology; Kemiteknik och kemisk teknologi; Chemical technology and engineering; real-time PCR; YPCE medium; Enrichment PCR; DNA polymerases; PCR inhibitors; pre-PCR processing; Salmonella; Diagnostic PCR; Yersinia enterocolitica; Microbiology; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi;

    Sammanfattning : This thesis deals with the methodological advances of diagnostic PCR including reliability of PCR and pre-PCR processing of food and feed samples. Diagnostic PCR has been greatly improved by the introduction of the second generation of PCR, so-called real-time PCR. LÄS MER

  3. 2. PCR inhibition mechanisms in forensic DNA analysis

    Författare :Maja Sidstedt; Teknisk mikrobiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; PCR inhibition mechanisms; Real-time PCR; Digital PCR; Massively parallel sequencing;

    Sammanfattning : In forensic DNA analysis, the polymerase chain reaction (PCR) enables DNAanalysis of minute biological crime scene traces. PCR is an enzymatic reaction for amplification of specific DNA fragments involving both biochemical andbiophysical processes. The main analytical challenge is posed by the nature of the samples of interest. LÄS MER

  4. 3. Multiplex HLA-DR-DQ genotyping : for genetic epidemiology and clinical risk assessment

    Författare :Ewa Lavant; Malmö högskola; []
    Nyckelord :HLA-DRB1; HLA-DQB1; HLA-DQA1; PCR-SSP; type 1 diabetes; celiac disease; autoimmune disease; capillary gel electrophoresis; genotyping;

    Sammanfattning : The human leukocyte antigens (HLA) are highly polymorphic cell surface proteins encoded in the major histocompatibility complex (MHC) region on chromosome 6. The HLA system has been well known as transplantation antigens but the primary biological role of the HLA molecules is regulation of immune response by presenting peptide fragments to T-lymphocytes. LÄS MER

  5. 4. On the Diagnosis and Management of Viral Respiratory Infections

    Författare :Robin Brittain-Long; Göteborgs universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Respiratory virus; Respiratory tract infection; Real-time PCR; Multiplex PCR; Antibiotic use;

    Sammanfattning : Acute respiratory tract infections (ARTIs), are the most common infections in man, and represent a major global health burden. Viruses, most often causing a mild and self-limiting disease, yet with substantial morbidity and high costs for society, mainly cause upper respiratory tract infections. LÄS MER

  7. 5. Selector Technology : For Multiplex DNA Analysis

    Författare :Fredrik Dahl; Mats Nilsson; Ulf Landegren; Ivo Glynne Gut; Uppsala universitet; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Genetic engineering; DNA technology; Genteknik; Genetic engineering including functional genomics; Genteknik inkl. funktionsgenomik;

    Sammanfattning : A majority of methods for identifying sequences in the human genome involve target sequence amplification through PCR. This work presents novel methods for amplifying circularized DNA and presents solutions for some major limitations of PCR. LÄS MER