Sökning: "Modified nucleoside"

Visar resultat 1 - 5 av 23 avhandlingar innehållade orden Modified nucleoside.

1. 1. Formation and function of wobble uridine modifications in transfer RNA of Saccharomyces cerevisiae

   Författare :Bo Huang; Anders Byström; Monica Rydén-Aulin; Umeå universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Transfer RNA; Modified nucleoside; Elongator complex; Wobble uridine; Decoding; Molecular biology; Molekylärbiologi;

   Sammanfattning : Transfer RNAs (tRNAs) act as adaptor molecules in decoding messenger RNA into protein. Frequently found in tRNAs are different modified nucleosides, which are derivatives of the four normal nucleosides, adenosine (A), guanosine (G), cytidine (C), and uridine (U). LÄS MER

3. 2. Novel Methods for Synthesis of High Quality Oligonucleotides

   Författare :Andrey Semenyuk; Marek Kwiatkowski; Ulf Landegren; Joachim W. Engels; Uppsala universitet; []
   Nyckelord :Organic chemistry; oligonucleotide; solid-phase synthesis; RNA synthesis; phosphoramidite; abasic sites; depurination; 2 -O-DTM; protecting group; cartridge; conjugate; dithiomethyl linker; base-stable linker; oligonucleotide purification; nucleoside; Organisk kemi;

   Sammanfattning : The first part of the work describes a procedure of oligonucleotide purification using a reversed-phase cartridge. The developed method employs a very efficient yet mild oligonucleotide detritylation on the cartridge support allowing fast purification of oligonucleotides regardless of their 5´-modification. LÄS MER

4. 3. Nucleic Acids as Drug Targets -Interactions of Platinum Complexes, Polyamines, and siRNA with DNA and RNA Models

   Författare :Margareta Hägerlöf; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Kinetics; Kemi; Chemistry; AU-rich region; siRNA; Circular Dichroism; Polyamine; Modified Nucleoside; HPLC; Thermodynamic Stability; cisplatin; Hairpin; DNA and RNA conformation; RNA; DNA; Anticancer Drug; Platinum Complexes;

   Sammanfattning : Drugs targeting nucleic acids were studied in DNA and RNA model systems. The effects were investigated with respect to structural and thermodynamic influence, kinetics, and biological function. The drugs employed in this thesis were anticancer active platinum(II) complexes, polyamine derivatives, and siRNA. LÄS MER

5. 4. Function of wobble nucleoside modifications in tRNAs of Salmonella enterica Serovar Typhimurium

   Författare :Peng Chen; Glenn R. Björk; Paul Agris; Umeå universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Molecular biology; tRNA; wobble nucleoside; frameshifting; translation; Molekylärbiologi; Molecular biology; Molekylärbiologi; molekylärbiologi; Molecular Biology;

   Sammanfattning : Transfer RNA from all organisms has modified nucleosides and position 34 (the wobble position) is one of the most extensively modified positions. Some wobble nucleoside modifications restrict codon choice (e.g. 5-methylaminomethyl-2-thiouridine, mnm5s2U) while some extend the decoding capacity (e. LÄS MER

7. 5. Conformationally Constrained Nucleosides : Design, Synthesis, and Biochemical Evaluation of Modified Antisense Oligonucleotides

   Författare :Oommen P. Varghese; Lars Engman; Jacek Stawinski; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Organic chemistry; chemically modified oligonucleotides; azetidine; aza-ENA-T; cyanomethylene locked; free energy of activation; diaxial interactions; chair conformation; stable duplex; human serum; snake venom phosphodiesterase; antisense agents; RNase H; Organisk kemi; Chemistry; Kemi;

   Sammanfattning : This thesis is concerned with synthesis, structure and biochemical analysis of chemically modified oligonucleotides with potential therapeutic applications. The three types of chemical modifications described here are: (a) A North-East locked 1',2'-azetidine nucleoside (b) A North locked 2',4'-cyanomethylene bridged nucleoside and (c) A 2',4'-aza-ENA-T nucleoside. LÄS MER