Sökning: "Minisequencing"
Visar resultat 1 - 5 av 13 avhandlingar innehållade ordet Minisequencing.
1. Using Minisequencing Technology for Analysing Genetic Variation in DNA and RNA
Sammanfattning : In this thesis, the four-color fluorescence tag-microarray minisequencing system pioneered by our group was further developed and applied for analysing genetic variation in human DNA and RNA. A SNP marker panel representing different chromosomal regions was established and used for identification of informative SNP markers for monitoring chimerism after stem cell transplantation (SCT). LÄS MER
2. Analysis of Nucleotide Variations in Non-human Primates
Sammanfattning : Many of our closest relatives, the primates, are endangered and could be extinct in a near future. To increase the knowledge of non-human primate genomes, and at the same time acquire information on our own genomic evolution, studies using high-throughput technologies are applied, which raises the demand for large amounts of high quality DNA. LÄS MER
3. Accessing Genetic Variation by Microarray Technology
Sammanfattning : Microarray technology is a promising approach for the simultaneous analysis of multiple single nucleotide polymorphisms (SNPs), which are the most abundant form of genetic variation. In this thesis enzyme-assisted microarray-based methods were developed to improve the accuracy and genotype discrimination power of the current methods for SNP genotyping. LÄS MER
4. Evaluation of New Technologies for Forensic DNA Analysis
Sammanfattning : DNA samples from crime scenes or mass disasters are often limited and degraded which limits the possibility of successful traditional STR analysis. Moreover, there is a need to decrease the turnaround time in criminal investigations. LÄS MER
5. Large-Scale Genotyping for Analysis of the Type I Interferon System in Autoimmune Diseases
Sammanfattning : Single nucleotide polymorphisms (SNPs) are the most common form of genetic variation. We developed a novel multiplexed method for SNP genotyping based on four-color fluorophore tag-microarray minisequencing. This method allows simultaneous genotyping of 80 samples and up to 200 SNPs in any allele combination. LÄS MER