Sökning: "Microfluidic CD"

Hittade 5 avhandlingar innehållade orden Microfluidic CD.

  1. 1. Compact-disc microfluidic methods for characterization of therapeutic antibodies : Analysis of post-translational modifications

    Författare :Thi Thuy Tran; Gunnar Thorsén; McLean John; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Microfluidic CD; therapeutic antibodies; post-translational modifications; glycosylation profiling; multivariate statistical analysis; MALDI-MS; Analytical Chemistry; analytisk kemi;

    Sammanfattning : Characterization of post-translational modifications (PTMs) of therapeutic proteins is very important during the bioprocess development to maintain desired product quality and during the submission process to regulatory authorities for product approval. Monitoring glycosylation in pharmacokinetic studies can be useful to evaluate the dependence of clearance rates on different glycoforms. LÄS MER

  3. 2. Sample preparation and mass spectrometry in proteome studies

    Författare :Daniel Hirschberg; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Proteome studies aim to link gene to function. Instead of screening for function in a specific protein as in classical protein chemistry, proteins are now screened against a specific phenotype, disease or metabolic state. LÄS MER

  4. 3. Development and application of alternative methods for profiling proteins N-glycosylation

    Författare :Alessandro Quaranta; Leopold Ilag; Niclas Karlsson; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; N-glycosylation; Glycomics; Glycosylation Biomarkers; Intact Glycoproteins; Glycoform Quantification; Mass Spectrometry; Ion Mobility Spectrometry; MALDI-MS; Paper Spray Ionization; Microfluidics; Magnetic Beads; Immunoaffinity Purification; Nanobodies; Analytical Chemistry; analytisk kemi;

    Sammanfattning : Glycosylation is a post-translational modification (PTM) that exerts profound structural and functional effects on the modified protein. Glycan synthesis and conjugation to proteins are regulated by a myriad of factors, both genetic and environmental, and are also influenced by external stressors. LÄS MER

  5. 4. Proteomic analyses of bioactive molecules in health and disease

    Författare :Theres Jägerbrink; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : After the mapping of genomes and knowing the blueprints of the possible gene products, focus is shifting from genomics to proteomics. Proteome analyses can be used for diagnosis, prognosis and monitoring of diseases and for characterization of bioactive compounds and their mechanisms of action. LÄS MER

  7. 5. Centrifugal microfluidics-based point of care diagnostics at resource limited settings

    Författare :Ahmad Saleem Akhtar; Aman Russom; Ines Fernandes Pinto; Nicole Pamme; KTH; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; microfluidics; centrifugal microfluidics; point-of-care; low-cost; diagnostics; agarose beads; immunoassays; colorimetry; fluorescence; cytokines; nucleic acid amplification; isothermal amplification; COVID-19; portable; smartphone; resource limited settings.; Biotechnology; Bioteknologi;

    Sammanfattning : Advancements in medical diagnostics have allowed us to understand the underlying mechanism and treat the root cause for many diseases which had been causing morbidity and mortality up until this point in human history. Furthermore, many of the standard diagnostic procedures have now been transformed to provide answers at or near the point-of-care. LÄS MER